發(fā)布時(shí)間：2018-03-02 21:18

  本文選題：溶酶體　切入點(diǎn)：熒光探針　出處：《有機(jī)化學(xué)》2017年02期 　論文類型：期刊論文

【摘要】：溶酶體在細(xì)胞各種生命活動(dòng),如物質(zhì)代謝、細(xì)胞膜循環(huán)、細(xì)胞凋亡中發(fā)揮著重要作用.將溶酶體可視化,并對(duì)其活性物種、特定微環(huán)境及關(guān)鍵生理過程進(jìn)行檢測(cè),不僅有助于理解溶酶體參與生命活動(dòng)的分子機(jī)制,而且對(duì)疾病的治療具有重要的指導(dǎo)意義.近年來,許多靶向溶酶體的熒光探針被相繼報(bào)道,其檢測(cè)對(duì)象包括還原性物種、活性氧、活性氮、氫離子、金屬離子、陰離子以及生物酶等,還包括對(duì)溫度、粘度、極性、p H等微環(huán)境的檢測(cè).溶酶體的熒光探針的定位機(jī)制分為三類:利用溶酶體的酸性生理環(huán)境、利用溶酶體的物質(zhì)代謝功能以及利用溶酶體特殊的膜蛋白或水解酶.對(duì)近年來出現(xiàn)的主要溶酶體熒光探針進(jìn)行了總結(jié)與評(píng)述,展望了溶酶體熒光探針應(yīng)用于探究重要細(xì)胞生理過程的分子機(jī)制,以及相關(guān)疾病診斷方面的發(fā)展趨勢(shì).
[Abstract]:Lysosomes play an important role in various cell life activities, such as substance metabolism, cell membrane circulation and apoptosis. The lysosomes are visualized and detected for their active species, specific microenvironments and key physiological processes. Not only is it helpful to understand the molecular mechanism of lysosome's involvement in life, but it is also of great significance in the treatment of diseases. In recent years, a number of fluorescent probes targeting lysosomes have been reported, including the detection of reductive species. Reactive oxygen species, active nitrogen, hydrogen ions, metal ions, anions, biological enzymes, and so on, including temperature, viscosity, The localization mechanism of lysosomal fluorescence probe is divided into three categories: using the acidic physiological environment of lysosome, Based on the metabolic function of lysosome and the special membrane protein or hydrolase of lysosome, the main lysosomal fluorescent probes in recent years were summarized and reviewed. The application of lysosomal fluorescence probe to the molecular mechanism of important cellular physiological processes and the development trend of diagnosis of related diseases are prospected.
【作者單位】： 北京師范大學(xué)化學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(No.21272027)資助項(xiàng)目~~
【分類號(hào)】：O657.3

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