本文關(guān)鍵詞： 高效液相色譜 熒光檢測 柑橘果汁 多甲氧基黃酮 香豆素　出處：《食品科學(xué)》2017年20期 　論文類型：期刊論文

【摘要】：建立高效液相色譜-串聯(lián)二極管陣列檢測器和熒光檢測器(high performance liquid chromatography-diode array detector-fluorescence detector,HPLC-DAD-FLD)同時檢測柑橘果汁中12種多甲氧基黃酮、香豆素及呋喃香豆素類物質(zhì)的方法。以0.01%磷酸、甲醇、乙腈組成三元流動相進行梯度洗脫,12種物質(zhì)在30 min內(nèi)實現(xiàn)基線分離。利用DAD和FLD獲得各物質(zhì)的紫外和熒光光譜信息,將柑橘果汁樣品組分的光譜與之比對,并結(jié)合色譜保留時間,實現(xiàn)樣品成分的定性分析。分別以320 nm和450 nm為紫外和熒光的檢測波長,研究該方法的定量性能。結(jié)果顯示:標準曲線線性關(guān)系良好;熒光定量限低至μg/L級,可作為紫外定量的重要補充手段;果汁回收率的紫外和熒光檢測值分別為95.2%~104.8%和94.5%~103.5%。該方法對樣品定性、定量分析準確,適合柑橘多甲氧基黃酮、香豆素等能發(fā)射熒光物質(zhì)的檢測。
[Abstract]:A method for simultaneous determination of 12 polymethoxyflavones, coumarins and furancoumarins in citrus juice by high performance liquid chromatography-diode array detector-fluorescence detector high performance liquid chromatography-diode array detector-fluorescence detector and fluorescence detector was established. A ternary mobile phase consisting of acetonitrile was used to carry out gradient elution of 12 substances in a baseline within 30 min. The UV and fluorescence spectra of each substance were obtained by DAD and FLD, and the spectra of citrus juice samples were compared with each other. The quantitative properties of the method were studied by using 320 nm and 450 nm as the detection wavelengths of UV and fluorescence, respectively. The results showed that the linear relationship of the standard curve was good. The fluorescence quantitative limit is as low as 渭 g / L and can be used as an important supplementary method for UV quantification. The UV and fluorescence detection values of the recovery rate of juice are 95.2% 104.8% and 94.5% 103.5%, respectively. This method is accurate for the qualitative and quantitative analysis of the samples and is suitable for citrus polymethoxy flavonoids. Coumarin and other emission fluorescent substances detection.
【作者單位】： 西南大學(xué)柑桔研究所中國農(nóng)業(yè)科學(xué)院柑桔研究所;重慶第二師范學(xué)院重慶市功能性食品協(xié)同創(chuàng)新中心重慶市功能性食品工程技術(shù)研究中心;西南大學(xué)食品科學(xué)學(xué)院;重慶第二師范學(xué)院生物與化學(xué)工程學(xué)院;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項(201503142-12) 重慶市功能性食品協(xié)同創(chuàng)新中心建設(shè)項目(167001);重慶市功能性食品協(xié)同創(chuàng)新中心科研項目(ccicff-rp-201604) 中央高�；究蒲袠I(yè)務(wù)費專項(XDJK2016E048) 重慶市統(tǒng)籌城鄉(xiāng)教師教育研究中心2015年工作室項目(JDGZS201507) 重慶第二師范學(xué)院2016年校級大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(201605)
【分類號】：O657;TS255.44
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本文編號：1531771