本文關(guān)鍵詞： 牛油果 內(nèi)部萃取電噴霧電離質(zhì)譜 化學(xué)成分 成熟程度　出處：《高等學(xué)�；瘜W(xué)學(xué)報(bào)》2017年05期 　論文類型：期刊論文

【摘要】：采用內(nèi)部萃取電噴霧電離質(zhì)譜(iEESI-MS)技術(shù),以甲醇作為萃取溶劑,無需樣品預(yù)處理,在優(yōu)化條件下分別對(duì)牛油果果肉和果皮中化學(xué)成分進(jìn)行快速直接鑒定,并考察了不同成熟度牛油果果肉中化學(xué)成分的差異.實(shí)驗(yàn)結(jié)果表明,在負(fù)離子檢測(cè)模式下,從果肉中鑒定出棕櫚酸、硬脂酸、棕櫚油酸、油酸和亞麻酸等12種有效營養(yǎng)成分,從果皮中鑒定出兒茶素、綠原酸等11種營養(yǎng)成分.利用正交偏最小二乘判別分析法(OPLS-DA)對(duì)不同成熟度的牛油果果肉的iEESI-MS指紋譜圖數(shù)據(jù)進(jìn)行分析發(fā)現(xiàn),此方法能夠有效判別不同成熟程度的牛油果且5種化學(xué)成分差異顯著.本方法無需樣品預(yù)處理、樣品耗量少、分析速度快(單個(gè)樣品檢測(cè)時(shí)間小于1 min)且操作簡便,為植物有效營養(yǎng)成分和醫(yī)用價(jià)值的開發(fā)提供了一種快速質(zhì)譜分析新方法.
[Abstract]:The internal extraction electrospray ionization mass spectrometry (iEESI-MSS) technique was used to identify the chemical constituents of avocado pulp and pericarp with methanol as extraction solvent and without sample pretreatment. The results showed that palmitic acid, stearic acid, palmitic oil acid, oleic acid and linolenic acid were identified from the pulp under the negative ion detection mode. Eleven nutritive components, such as catechin and Lv Yuan acid, were identified from pericarp. The iEESI-MS fingerprint data of avocado pulp with different maturity were analyzed by orthogonal partial least squares discriminant analysis (OPLS-DA). This method can effectively distinguish avocados of different maturation degree and the difference of five chemical components is significant. This method does not need the sample pretreatment, the sample consumption is less, the analysis speed is fast (the detection time of a single sample is less than 1 min), and the operation is simple and convenient. A new rapid mass spectrometry analysis method is provided for the development of plant effective nutrients and medical value.
【作者單位】： 吉林大學(xué)化學(xué)學(xué)院無機(jī)合成與制備化學(xué)國家重點(diǎn)實(shí)驗(yàn)室;東華理工大學(xué)江西省質(zhì)譜科學(xué)與儀器重點(diǎn)實(shí)驗(yàn)室;吉林大學(xué)環(huán)境與資源學(xué)院地下水資源與環(huán)境教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(批準(zhǔn)號(hào):21307040,21427802) 江西省質(zhì)譜科學(xué)與儀器重點(diǎn)實(shí)驗(yàn)室開放基金(批準(zhǔn)號(hào):JXMS201601)資助~~
【分類號(hào)】：O657.63;TS255.7
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本文編號(hào)：1530462