發(fā)布時(shí)間：2018-02-12 23:16

  本文關(guān)鍵詞： 微乳液相色譜法 降壓 中成藥 保健品 化學(xué)藥物　出處：《色譜》2017年02期 　論文類型：期刊論文

【摘要】：建立了微乳液相色譜(MELC)同時(shí)測定中成藥及保健品中添加的10種降壓藥物的分析方法�？疾炝宋⑷榱鲃�(dòng)相的組成成分(包括表面活性劑、助表面活性劑、油相的種類及質(zhì)量分?jǐn)?shù))、pH值和固定相種類對(duì)分離效果的影響。同時(shí)利用星點(diǎn)設(shè)計(jì)-響應(yīng)面優(yōu)化法進(jìn)一步優(yōu)化微乳組成。實(shí)驗(yàn)得到的最佳分離條件:色譜柱為Agela Promosil C18(2)(250 mm×4.6 mm,5μm);微乳流動(dòng)相為5.6%(質(zhì)量分?jǐn)?shù))十二烷基聚乙二醇醚(Brij35)-0.17%(質(zhì)量分?jǐn)?shù))十二烷基硫酸鈉(SDS)-5.1%(質(zhì)量分?jǐn)?shù))正丁醇-0.46%(質(zhì)量分?jǐn)?shù))環(huán)己烷-水(pH 3.0);流速為1.0mL/min;檢測波長為230 nm;柱溫為30℃。該方法具有靈敏度高、穩(wěn)定性好和環(huán)保的優(yōu)點(diǎn)。
[Abstract]:A method for simultaneous determination of 10 antihypertensive drugs in Chinese patent medicine and health products by microemulsion liquid chromatography (MELC) was established. The composition of mobile phase of microemulsion (including surfactant and cosurfactant) was investigated. The effects of oil phase types, mass fraction, pH value and stationary phase species on the separation effect were studied. The composition of microemulsion was further optimized by star design-response surface optimization method. The optimum separation conditions were obtained as follows: the chromatographic column was Agela Promosil. The mobile phase of microemulsion was 5.6% (mass fraction) of 12 alkylpolyethyleneglycol ether, and 12 (mass fraction) of sodium alkyl sulfate (mass fraction) 12 alkyl sodium sulfate (mass fraction), n-butanol -0.46 (mass fraction) cyclohexane-water pH 3.0, flow rate 1.0 mL / min, detection wavelength 230nm; The column temperature is 30 鈩，

本文編號(hào)：1506787