本文關鍵詞： 超聲細胞粉碎提取 響應曲面法 六味地黃丸 質(zhì)量控制　出處：《色譜》2017年01期 　論文類型：期刊論文

【摘要】：針對中藥復方制劑六味地黃丸的質(zhì)量控制提出了一種多指標全藥材整體分析新策略。從組成六味地黃丸的每一味藥材中選取1~2個藥效成分,即選取熟地黃中的毛蕊花糖苷、山茱萸中的莫諾苷和馬錢苷、牡丹皮中的丹皮酚和芍藥苷、山藥中的尿囊素、茯苓中的茯苓酸、澤瀉中的23-乙酰澤瀉醇B作為六味地黃丸的質(zhì)量控制指標成分,采用快速、簡便、高效的超聲細胞粉碎提取(UCGE)法對這8個指標成分進行同步提取。用響應曲面分析中的Box-Behnken模式對UCGE提取過程中對提取效率影響較大的料液比、提取時間和提取溫度等參數(shù)進行了優(yōu)化。得到最優(yōu)的提取條件:料液比1∶45(g/mL)、提取時間40 min、提取溫度20℃。進一步利用超高效液相色譜-二極管陣列檢測(UPLC-PAD)技術實現(xiàn)多指標成分在同一根色譜柱上的同步分離和檢測,從而建立六味地黃丸的多指標全藥材整體色譜。采用HSS T3色譜柱(50 mm×2.1 mm,1.8μm),以乙腈-水為流動相進行梯度洗脫,基于指標成分的紫外光譜特征選擇5個檢測波長同時檢測,并進行了線性關系、檢出限、定量限、精密度、穩(wěn)定性、重復性、準確性等方法學驗證。該研究為六味地黃丸質(zhì)量標準的建立提供了依據(jù),同時也為中藥復方制劑提供了一種質(zhì)量控制新模式和新理念。
[Abstract]:According to the quality control of Liuwei Dihuang pills, a new strategy for the whole analysis of multi-index whole medicinal materials was put forward. One or two effective components were selected from each drug of Liuwei Dihuang pills, that is, Ruirin in Rehmannia Rehmanniae was selected. Monoside and marganin in Cornus officinalis, paeonol and paeoniflorin in peony peel, allantoin in yam, Poria cocos in Poria cocos, and 23- acetylalisin B in alisma alisma were used as quality control index components of Liuwei Dihuang pills. The eight index components were extracted synchronously by a simple and efficient ultrasonic cell crushing extraction method. The ratio of material to liquid had a great influence on the extraction efficiency of UCGE by using the Box-Behnken pattern in response surface analysis. The extraction time and temperature were optimized, and the optimal extraction conditions were obtained: the ratio of material to liquid was 1: 45 g / mL, the extraction time was 40 min, and the extraction temperature was 20 鈩，

本文編號：1502531