本文關(guān)鍵詞： 廣豆根 黃酮類 黑升麻 皂苷類 分離鑒定　出處：《西南科技大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

【摘要】：廣豆根及黑升麻具有抗腫瘤、抗炎等藥理功效,是重要的傳統(tǒng)中藥材。廣豆根所含的黃酮類成分具有較好的抗?jié)兓钚院秃谏樗脑碥粘煞志哂休^好的緩解更年期作用。本文對廣豆根的黃酮類成分和黑升麻皂苷成分進(jìn)行研究,為藥理活性和構(gòu)效關(guān)系奠定基礎(chǔ)。采用硅膠柱色譜、葡聚糖凝膠色譜Sephadex LH-20、反相柱色譜及高效液相色譜(High Performance Liquid Chromatography,HPLC)等色譜技術(shù),并結(jié)合結(jié)晶與重結(jié)晶等分離手段,對廣豆根進(jìn)行分離純化。采用質(zhì)譜(Mass Spectrometry,MS)、核磁共振波譜(Nuclear Magnetic Resonance Spectroscopy,NMR)、紫外光譜(Ultraviolet Absorption Spectroscopy,UV)等現(xiàn)代波譜學(xué)技術(shù),對分離到的化合物進(jìn)行結(jié)構(gòu)鑒定。采用色譜技術(shù),從廣豆根的二氯甲烷部分,分離到11個(gè)化合物。經(jīng)波譜學(xué)技術(shù),鑒定出9個(gè)黃酮類化合物:廣豆根素(1,1.300 g)、多花胡枝子素A3(2,0.810 g)、L-高麗槐素(3,0.325 g)、光甘草酚(4,0.056 g)、廣豆根酮(7,0.051 g)、山豆根色烯素(8,0.750 g)、Lupinifolin(9,0.015 g)、5-Hydroxysophoranone(10,0.18 g),同時(shí)還得到3個(gè)非黃酮類化合物:β-谷甾醇(5,0.098 g)羽扇豆醇(6,0.043 g)和大豆皂醇B(11,0.010 g)�；衔�2為首次從該屬植物中分離得到,化合物10為首次從該植物中分離得到。采用反復(fù)柱色譜和重結(jié)晶等方法對黑升麻的乙酸乙酯部位進(jìn)行分離純化,從黑升麻中分離得到黑升麻苷(0.208 g)和27位脫氧黑升麻苷(0.017 g),純度分別為98.2%和98.3%,產(chǎn)率分別是0.020%和0.001%。
[Abstract]:Gangdou root and black cohosh have anti-tumor, anti-inflammatory and other pharmacological effects. It is an important traditional Chinese medicinal material. The flavonoids contained in the root of Guangdou have better anti-ulcer activity and the saponins contained in the black cohosh have a better effect on relieving climacteric. The components of saponins were studied. For the basis of pharmacological activity and structure-activity relationship, silica gel column chromatography and dextran gel chromatography (Sephadex LH-20) were used. Reversed phase column chromatography (RPC) and high performance liquid chromatography (HPLC) with high Performance Liquid chromatographyHPLC. In combination with crystallization and recrystallization, the root was separated and purified by mass spectrometric mass spectrometry (MS). Nuclear Magnetic Resonance spectroscopy (NMRs). Ultraviolet Absorption Spectroscopy (UV) and other modern spectroscopic techniques. 11 compounds were isolated from the dichloromethane part of Gangdou root by chromatographic technique, and 9 flavonoids were identified by spectroscopic technique. 1.300 g / g, 0.300 g / g, 0.810 g / L, 0.325 g / L, 0.056 g / L, 0.056 g / L, 7g / L, respectively. 0. 051 g / g, Lupinifolin9 (0. 015 g). 5-Hydroxysophoranoneum 100.18 g, and three non-flavonoids, 尾 -sitosterol 50.098 g) were also obtained. Compound 2 was isolated from this genus for the first time. Compound 10 was isolated from the plant for the first time. Repeated column chromatography and recrystallization were used to isolate and purify ethyl acetate from black cohosh. The purity was 98.2% and 98.3%, respectively. The yields were 0.020% and 0.001, respectively.
【學(xué)位授予單位】：西南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R284;O657.7

【相似文獻(xiàn)】

相關(guān)會(huì)議論文 前2條

1 白文佩;王淑玉;劉建立;耿力;胡麗娜;habil;Eckehard Liske H.-H;Henneicke-von Zepelin;;黑升麻與7-甲基異炔諾酮治療更年期癥狀的療效和安全性比較[A];第二次全國絕經(jīng)相關(guān)問題高級(jí)學(xué)術(shù)研討會(huì)論文集[C];2006年

2 謝梅青;李揚(yáng)志;洪宇;陳冬梅;李春花;;黑升麻異丙醇提取物改善GnRHa療程中低雌激素癥狀的研究[A];中華醫(yī)學(xué)會(huì)第三次全國絕經(jīng)學(xué)術(shù)會(huì)議暨絕經(jīng)相關(guān)問題學(xué)習(xí)班論文匯編[C];2011年

相關(guān)重要報(bào)紙文章 前3條

1 江蘇省藥品不良反應(yīng)監(jiān)測中心;黑升麻與肝毒性[N];健康報(bào);2006年

2 董江萍;英澳發(fā)現(xiàn)黑升麻引起肝損害[N];中國醫(yī)藥報(bào);2005年

3 盛文;EMEA評(píng)價(jià)認(rèn)為黑升麻與肝損害相關(guān)[N];中國醫(yī)藥報(bào);2006年

相關(guān)博士學(xué)位論文 前1條

1 吳飛;戊酸雌二醇和黑升麻異丙醇提取物對卵巢切除小鼠的影響及其機(jī)制探討[D];北京協(xié)和醫(yī)學(xué)院;2016年

相關(guān)碩士學(xué)位論文 前3條

1 東靜靜;黑升麻提取物對去勢大鼠血清性激素及相關(guān)靶器官ER表達(dá)水平的影響[D];華北理工大學(xué);2015年

2 楊婷;廣豆根黃酮類成分及黑升麻皂苷成分的研究[D];西南科技大學(xué);2016年

3 南士濱;黑升麻功效的作用機(jī)理研究[D];上海師范大學(xué);2013年

，

本文編號(hào)：1489139