本文關(guān)鍵詞： 超高效液相色譜-三重四極桿-線性離子阱質(zhì)譜(Qtrap-UPLC-MS/MS) 礦物藥 青礞石 癲癇 氨基酸遞質(zhì) 海馬 皮層　出處：《質(zhì)譜學(xué)報(bào)》2016年06期 　論文類型：期刊論文

【摘要】：建立了超高效液相色譜-三重四極桿-線性離子阱質(zhì)譜(Qtrap-UPLC-MS/MS)法同時(shí)測(cè)定大鼠腦組織中4種氨基酸類神經(jīng)遞質(zhì),研究了礦物藥青礞石原藥材粉末、水煎液、藥渣對(duì)戊四氮(Pentylenetetrazol,PTZ)點(diǎn)燃癲癇模型大鼠腦組織中氨基酸類神經(jīng)遞質(zhì)含量的影響。在ESI+電離模式下,采用多反應(yīng)監(jiān)測(cè)掃描方式測(cè)定腦組織(皮層,海馬)中谷氨酸(Glu)、甘氨酸(Gly)、天冬氨酸(Asp)及γ-氨基丁酸(GABA)的含量。結(jié)果表明:4種氨基酸的線性關(guān)系良好,精密度、準(zhǔn)確度均符合生物樣品的分析要求。大鼠給予青礞石不同樣品后,腦組織皮層和海馬中興奮性神經(jīng)遞質(zhì)Glu、Asp水平顯著降低。與模型組相比,皮層中Gly占氨基酸總含量的百分比均增加,Asp/Gly值、Glu/Gly值均降低;海馬中,各組Glu、Asp、Gly、GABA的濃度均降低。這說明,礦物藥青礞石能有效降低PTZ點(diǎn)燃大鼠腦內(nèi)興奮性氨基酸遞質(zhì)含量,調(diào)節(jié)皮層和海馬區(qū)Gly含量。
[Abstract]:A high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry (Qtrap-UPLC-MS / MS) method was developed for simultaneous determination of four amino acid neurotransmitters in rat brain. In this paper, we studied the effect of the mineral drug, protophore powder, decoction and residue on pentylenetetrazol (Pentylenetetrazol) in pentylenetetrazol (PTZ). The effect of amino acid neurotransmitters in brain tissue of kindled epileptic rats was studied. Under ESI ionization mode, the brain tissue (cortex) was measured by multi-reaction monitoring scanning. The contents of glutamate, glycine, aspartic acid and GABA in hippocampus showed a good linear relationship and precision. The level of excitatory neurotransmitter Gluus Asp in cerebral cortex and hippocampus of rats was significantly lower than that of model group. The percentage of Gly in total amino acid content in cortex increased and the value of Asp-gly decreased. In the hippocampus, the concentration of Glu-AspGlyGA-GABA in each group was decreased, which indicated that the mineral penicillin could effectively reduce the content of excitatory amino acid transmitters in the brain of PTZ kindled rats. To regulate the content of Gly in cortex and hippocampus.
【作者單位】： 南京中醫(yī)藥大學(xué)藥學(xué)院江蘇省中藥資源產(chǎn)業(yè)化過程協(xié)同創(chuàng)新中心;南京中醫(yī)藥大學(xué)信息技術(shù)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(81303178) 江蘇省自然基金項(xiàng)目(BK20130957) 江蘇省高校優(yōu)勢(shì)學(xué)科建設(shè)工程資助項(xiàng)目(二期) 江蘇高校品牌專業(yè)建設(shè)工程項(xiàng)目(PPZY2015A070)資助
【分類號(hào)】：O657.63;R285.5
【正文快照】： 青礞石(Chloriti Lapis)為變質(zhì)巖類黑云母片巖(Biotite Schist)或綠泥石化云母碳酸鹽片巖(Mica Carbonate Schist by Chloritization),是一種主要含K、Mg、Fe、Al等元素的硅酸鹽[K(Mg·Fe)2(A1Si3O10)(OH,F)2],其中Si、Fe、Mg、Al、Ca、K、Na等7種元素為主要成分,累積貢獻(xiàn)率達(dá)

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