穗花狐尾藻內(nèi)生細(xì)菌的溶磷特征及其對(duì)宿主植物修復(fù)水體的作用
發(fā)布時(shí)間：2018-12-26 10:41
【摘要】：目前,水體富營(yíng)養(yǎng)化是我國(guó)自然水體面臨的最為嚴(yán)重的污染問題。為治理水體富營(yíng)養(yǎng)化已采用過物理、化學(xué)等多種方法,但都存在一些弊端,當(dāng)前生物修復(fù)水體富營(yíng)養(yǎng)化的方法有較大優(yōu)勢(shì),應(yīng)用前景廣闊,生物修復(fù)中的植物、微生物修復(fù)富營(yíng)養(yǎng)化水體為新興的較有效的治理方法。植物修復(fù)是近年來發(fā)展起來的一種效果好、耗能低、成本低廉的治理富營(yíng)養(yǎng)化水體的新技術(shù),但北方水體中植物生長(zhǎng)周期比較短,且植物對(duì)水體及底泥中的磷吸收較少,為克服水生植物在修復(fù)水體富營(yíng)養(yǎng)化過程中遇到的以上難題,從水生植物中分離出具有促生并溶磷作用的植物內(nèi)生菌與水生植物聯(lián)合作用治理水體富營(yíng)養(yǎng)化將是具有廣闊應(yīng)用前景的生物修復(fù)技術(shù)。本研究利用從穗花狐尾藻植株中分離篩選出具有溶磷作用的四株植物內(nèi)生細(xì)菌并通過溶磷能力比較實(shí)驗(yàn)篩選出具有較高應(yīng)用價(jià)值的溶磷內(nèi)生細(xì)菌W3,初步探討了其生長(zhǎng)最適培養(yǎng)條件和在不同培養(yǎng)條件下的溶磷特性,并在應(yīng)用模擬實(shí)驗(yàn)中對(duì)內(nèi)生溶磷菌株W3的促生潛力、對(duì)底泥中不同形態(tài)磷的釋放作用及其對(duì)宿主植物穗花狐尾藻修復(fù)水體的促進(jìn)作用做了初步研究。主要研究結(jié)果如下:從穗花狐尾藻中分離出若干株植物內(nèi)生菌,并作溶磷實(shí)驗(yàn)初步篩選出四株具有溶磷作用的植物內(nèi)生細(xì)菌,對(duì)四株內(nèi)生細(xì)菌做幾項(xiàng)溶磷特征比較實(shí)驗(yàn),分別測(cè)定四株內(nèi)生細(xì)菌在溶磷培養(yǎng)基中培養(yǎng)48h、96h、144h后上清液中正磷酸鹽濃度,以溶磷效果較佳的菌株W3為例,三次采樣時(shí)間測(cè)得正磷酸鹽濃度分別為56.16、269.60、282.05mg/L(對(duì)照組分別為5.98、5.62、6.05mg/L),由于96h增加量213.44mg/L顯著高于144h增加量12.45mg/L,結(jié)果表明96h達(dá)到較佳溶磷效果;研究不同磷酸鹽(卵磷脂、磷酸鋁、磷酸鈣)對(duì)菌株溶磷效果的影響,結(jié)果表明四株菌株溶解磷酸鈣效果最好,對(duì)照組與加菌實(shí)驗(yàn)組的正磷酸鹽濃度分別為25.62、157.07、158.79、239.60、58.84mg/L,篩選出優(yōu)勢(shì)溶磷菌株W3;在不同碳氮源對(duì)菌株溶磷效果比較實(shí)驗(yàn)研究中得出四株內(nèi)生細(xì)菌在以葡萄糖為碳源、以硫酸銨為氮源的培養(yǎng)基中溶磷效果最佳,W3溶磷能力最強(qiáng),其正磷酸鹽濃度為499.09mg/L。研究表明菌株W3適宜生長(zhǎng)的pH值范圍為5.0-9.0,改變無機(jī)磷基礎(chǔ)培養(yǎng)基中碳氮比對(duì)菌株W3的溶磷效果有影響,菌株W3在培養(yǎng)基初始pH值為7.0時(shí)溶磷效果最佳,發(fā)酵液pH值的變化與磷酸鈣的溶解明顯相關(guān),菌株W3吲哚乙酸和鐵載體的產(chǎn)量較高,具有一定促進(jìn)植物生長(zhǎng)的潛力,并且對(duì)底泥中不同形態(tài)的磷有一定的釋放作用。內(nèi)生溶磷細(xì)菌W3具有促進(jìn)底泥中磷的轉(zhuǎn)化作用,并在促進(jìn)宿主植物穗花狐尾藻生長(zhǎng)的同時(shí)提高了宿主植物對(duì)水體的修復(fù)效果。
[Abstract]:At present, eutrophication is the most serious pollution problem in China. In order to control eutrophication of water body, many methods have been used, such as physics and chemistry, but there are some disadvantages. Microbial remediation of eutrophication water is a new and effective treatment method. Phytoremediation is a new technique to treat eutrophication water with good effect, low energy consumption and low cost in recent years. However, the plant growth period in the northern water body is relatively short, and the plant absorbs less phosphorus in the water body and sediment. In order to overcome the above problems encountered by aquatic plants in the process of remediation of eutrophication, It will be a promising bioremediation technique to isolate plant endophytes which can promote growth and dissolve phosphorus from aquatic plants and combine them with aquatic plants to control eutrophication of water body. In this study, four endophytic bacteria with phosphorus dissolving activity were isolated and screened from the plants of Cercospora pumila, and the endophytic bacteria W3 with high application value were screened by comparison of phosphorus solubilizing ability. The optimum conditions for growth and the characteristics of phosphorus solubilization under different culture conditions were discussed, and the potential to promote the growth of endophytic phosphorus soluble strain W3 was studied in simulated experiments. In this paper, the release of phosphorus from sediment and its promoting effect on the recovery of water body by the host plant, Phaeopsis sphaeracea, were preliminarily studied. The main results are as follows: a number of endophytic bacteria were isolated from Sargassum spp. Four endophytic bacteria with phosphorus dissolving activity were screened by phosphorus solubilization experiment, and four endophytic bacteria were compared with other endophytic bacteria. The concentration of orthophosphate in the supernatant of four endophytic bacteria cultured in phosphate solubilizing medium for 48h, 96h and 144h respectively was determined. The strain W3, which had better phosphorus dissolution effect, was taken as an example. The concentration of orthophosphate measured at three sampling times was 56.16269.60282.05mg/L (the control group was 5.98% 5.62 mg / L). The increase of 213.44mg/L in 96h was significantly higher than that in 144h (12.45mg / L). The results showed that the phosphorus solubilization effect was better at 96 h. The effects of different phosphates (lecithin, aluminum phosphate, calcium phosphate) on the phosphorus dissolution of the strain were studied. The results showed that the four strains had the best effect on the dissolution of calcium phosphate. The concentrations of orthophosphate in the control group and the experimental group were 25.62157.07158.79239.6058.84mg / L, respectively. The results showed that four endophytic bacteria with glucose as carbon source and ammonium sulfate as nitrogen source had the best phosphorus solubilization effect, and W3 had the strongest phosphorus solubilization ability in different carbon and nitrogen sources, and the results showed that four endophytic bacteria had the best phosphorus solubilizing effect in the medium with glucose as carbon source and ammonium sulfate as nitrogen source. Its orthophosphate concentration is 499.09 mg / L. The results showed that the pH value of strain W3 was 5.0-9.0. The effect of phosphorus solubilization of strain W3 was best when the initial pH value of the medium was 7.0, and the ratio of carbon to nitrogen in the inorganic phosphorus basic medium had an influence on the phosphorus solubilization of strain W3. The change of pH value in fermentation broth was obviously related to the dissolution of calcium phosphate. The yield of indoleacetic acid and iron carrier of strain W3 was higher, which had the potential to promote plant growth and release phosphorus of different forms in sediment. Endophytic phosphorus-soluble bacteria W3 can promote the transformation of phosphorus in sediment and promote the growth of host plant, and improve the effect of host plants on water remediation.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X52;X17
本文編號(hào)：2391977
[Abstract]:At present, eutrophication is the most serious pollution problem in China. In order to control eutrophication of water body, many methods have been used, such as physics and chemistry, but there are some disadvantages. Microbial remediation of eutrophication water is a new and effective treatment method. Phytoremediation is a new technique to treat eutrophication water with good effect, low energy consumption and low cost in recent years. However, the plant growth period in the northern water body is relatively short, and the plant absorbs less phosphorus in the water body and sediment. In order to overcome the above problems encountered by aquatic plants in the process of remediation of eutrophication, It will be a promising bioremediation technique to isolate plant endophytes which can promote growth and dissolve phosphorus from aquatic plants and combine them with aquatic plants to control eutrophication of water body. In this study, four endophytic bacteria with phosphorus dissolving activity were isolated and screened from the plants of Cercospora pumila, and the endophytic bacteria W3 with high application value were screened by comparison of phosphorus solubilizing ability. The optimum conditions for growth and the characteristics of phosphorus solubilization under different culture conditions were discussed, and the potential to promote the growth of endophytic phosphorus soluble strain W3 was studied in simulated experiments. In this paper, the release of phosphorus from sediment and its promoting effect on the recovery of water body by the host plant, Phaeopsis sphaeracea, were preliminarily studied. The main results are as follows: a number of endophytic bacteria were isolated from Sargassum spp. Four endophytic bacteria with phosphorus dissolving activity were screened by phosphorus solubilization experiment, and four endophytic bacteria were compared with other endophytic bacteria. The concentration of orthophosphate in the supernatant of four endophytic bacteria cultured in phosphate solubilizing medium for 48h, 96h and 144h respectively was determined. The strain W3, which had better phosphorus dissolution effect, was taken as an example. The concentration of orthophosphate measured at three sampling times was 56.16269.60282.05mg/L (the control group was 5.98% 5.62 mg / L). The increase of 213.44mg/L in 96h was significantly higher than that in 144h (12.45mg / L). The results showed that the phosphorus solubilization effect was better at 96 h. The effects of different phosphates (lecithin, aluminum phosphate, calcium phosphate) on the phosphorus dissolution of the strain were studied. The results showed that the four strains had the best effect on the dissolution of calcium phosphate. The concentrations of orthophosphate in the control group and the experimental group were 25.62157.07158.79239.6058.84mg / L, respectively. The results showed that four endophytic bacteria with glucose as carbon source and ammonium sulfate as nitrogen source had the best phosphorus solubilization effect, and W3 had the strongest phosphorus solubilization ability in different carbon and nitrogen sources, and the results showed that four endophytic bacteria had the best phosphorus solubilizing effect in the medium with glucose as carbon source and ammonium sulfate as nitrogen source. Its orthophosphate concentration is 499.09 mg / L. The results showed that the pH value of strain W3 was 5.0-9.0. The effect of phosphorus solubilization of strain W3 was best when the initial pH value of the medium was 7.0, and the ratio of carbon to nitrogen in the inorganic phosphorus basic medium had an influence on the phosphorus solubilization of strain W3. The change of pH value in fermentation broth was obviously related to the dissolution of calcium phosphate. The yield of indoleacetic acid and iron carrier of strain W3 was higher, which had the potential to promote plant growth and release phosphorus of different forms in sediment. Endophytic phosphorus-soluble bacteria W3 can promote the transformation of phosphorus in sediment and promote the growth of host plant, and improve the effect of host plants on water remediation.
【學(xué)位授予單位】：吉林農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：X52;X17
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，本文編號(hào)：2391977
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