發(fā)布時(shí)間：2018-04-20 17:54

  本文選題：p-TSA + 施氏假單胞菌Y-2��； 參考：《南京農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：對甲苯磺酸(p-TSA)是一種廣泛應(yīng)用于工業(yè)中的重要的化工原料,含p-TSA的廢水大量排放到環(huán)境中,會(huì)對人類健康以及生態(tài)環(huán)境造成嚴(yán)重威脅。本論文從江蘇豐山制藥廠廢水處理池活性污泥中,通過富集、分離,篩選出一株能以p-TSA為唯一碳源生長的菌株,命名為Y-2。該菌株能夠在16h內(nèi)將200mg/L的p-TSA完全降解。經(jīng)鑒定,菌株Y-2為革蘭氏陰性菌,根據(jù)其生理生化特征以及16s rRNA基因序列同源性比較,菌株Y-2被鑒定為施氏假單胞菌(Pseudomonasstutzeri)。菌株Y-2在LB培養(yǎng)基中生長良好,0-5h菌株處于延滯期,5h開始,菌株進(jìn)入對數(shù)生長期,16h-25h菌株為穩(wěn)定期,25h后菌株逐漸衰亡。菌株Y-2生長的最適溫度為30°C,最適pH為7.0-8.0;菌株Y-2為嚴(yán)格好氧菌,通氣量越多,菌株生長越好;NaCl濃度也對菌株的生長有影響,菌株Y-2在NaCl濃度為5-15g/L時(shí)長勢較好,菌株生長最適的NaCl濃度為10g/L。降解菌Y-2可利用葡萄糖、果糖和麥芽糖等碳源,但是不能利用蔗糖、甘露醇、木糖等碳源;能利用蛋白胨、硫酸銨和氯化銨等氮源,也可以利用硝態(tài)氮,但不能利用尿素。菌株Y-2可以在16h之內(nèi)完全降解濃度為200mg/L的p-TSA。菌株降解p-TSA的最適pH為7.0-8.0,最適溫度為300°C;通氣量越大越有利于p-TSA的降解。接種量對菌株Y-2對p-TSA的降解也有影響,當(dāng)接種量在0.1%-2%的范圍內(nèi)時(shí),加大接種量可以明顯提高降解速率,接種量達(dá)到4%后,再增大接種量p-TSA降解速率基本不變。隨著p-TSA初始濃度的增加,菌株Y-2的延滯期越來越長,但菌株能夠在40h之內(nèi)完全降解500mg/L的p-TSA,降解效率較高。添加200mg/L的葡萄糖作為額外碳源會(huì)促進(jìn)p-TSA的降解,大大縮短降解時(shí)間。通過液質(zhì)聯(lián)用檢測p-TSA降解過程中的代謝產(chǎn)物,發(fā)現(xiàn)主要的代謝產(chǎn)物有對羧基苯磺酸、對羥基苯磺酸、對苯二酚、丁烯二酸和乙二酸。根據(jù)檢測結(jié)果推測可能的降解途徑為:首先是甲基側(cè)鏈氧化成羧基,緊接著羧基進(jìn)一步脫碳生成羥基,生成對羥基苯磺酸,然后磺酸基脫落變成羥基,生成對苯二酚,然后對苯二酚開環(huán),變成丁烯二酸和乙二酸,最后基本將其完全礦化。用勞麥方程對菌株Y-2降解p-TSA的降解動(dòng)力學(xué)進(jìn)行擬合,得出其動(dòng)力學(xué)參數(shù)分別為 Vmax =46.95 mg/L/h,Ks=59.09 mg/L,當(dāng)?shù)孜?p-TSA 濃度遠(yuǎn)遠(yuǎn)大于 46.95mg/L時(shí),菌株Y-2對p-TSA的降解符合零級動(dòng)力學(xué)模型,即p-TSA以最大速率降解,而與底物濃度無關(guān)。當(dāng)?shù)孜飌-TSA濃度遠(yuǎn)遠(yuǎn)小于46.95mg/L時(shí),此時(shí)對甲苯磺酸降解速率與底物濃度呈一級反應(yīng)關(guān)系,即底物濃度越高,降解速率越快。而在中間濃度時(shí),菌株Y-2對p-TSA的降解處于混合級反應(yīng)區(qū)。
[Abstract]:P-toluenesulfonic acid (p-TSA) is an important chemical raw material widely used in industry. Wastewater containing p-TSA is discharged into the environment in large quantities, which will pose a serious threat to human health and ecological environment. In this paper, a strain named Y-2 was selected from activated sludge of waste water treatment pond of Fengshan Pharmaceutical Factory in Jiangsu Province, which can grow with p-TSA as the sole carbon source by enrichment and separation. The strain could completely degrade the p-TSA of 200mg/L within 16 hours. The strain Y-2 was identified as a gram-negative strain. According to its physiological and biochemical characteristics and the homology of 16s rRNA gene sequence, strain Y-2 was identified as Pseudomonas Scheridoides. The strain Y-2 grew well in LB medium for 0-5h, and the strain began to decline after entering logarithmic growth stage (16h-25h) for 25 h. The optimum growth temperature and pH of strain Y-2 were 30 擄C and 7.0-8.0, respectively. Strain Y-2 was a strict aerobic bacterium, and the more aeration, the better the growth of strain Y-2, and the better the concentration of NaCl was, the better the growth of strain Y-2 was when the concentration of NaCl was 5-15g/L. The optimal concentration of NaCl for the growth of the strain was 10 g / L. The degradation bacteria Y-2 can use carbon sources such as glucose, fructose and maltose, but not sucrose, mannitol, xylose, nitrogen sources such as peptone, ammonium sulfate and ammonium chloride, but not urea. Strain Y-2 could completely degrade p-TSA with 200mg/L concentration within 16 hours. The optimum pH and temperature for p-TSA degradation were 7.0-8.0 and 300 擄C, respectively. The higher the aeration rate was, the more favorable the degradation of p-TSA was. The amount of inoculation also affected the degradation of p-TSA of strain Y-2. When the inoculation amount was in the range of 0.1-2%, the degradation rate of p-TSA could be increased obviously when the inoculation amount reached 4%, and the degradation rate of p-TSA was almost unchanged when the inoculation amount reached 4%. With the increase of initial concentration of p-TSA, the delay period of strain Y-2 was longer and longer, but the strain could completely degrade p-TSA of 500mg/L within 40 h, and the degradation efficiency was higher. The addition of 200mg/L as an additional carbon source could promote the degradation of p-TSA and shorten the degradation time. The main metabolites were p-carboxybenzenesulfonic acid, p-hydroxy benzenesulfonic acid, hydroquinone, butylenedioic acid and glycolic acid. According to the detection results, the possible degradation pathway is as follows: first, methyl side chain is oxidized to carboxyl group, then carboxyl group further decarbonizes to form p-hydroxy benzenesulfonic acid, then sulfonic group shedding to hydroxyl group to form hydroquinone. Then hydroquinone is ring-opening and becomes succinic acid and glycolic acid, and finally it is completely mineralized. The degradation kinetics of strain Y-2 degrading p-TSA was fitted by Laumai equation, and the kinetic parameters were obtained as follows: Vmax = 46.95 mg / L / L / L = 59.09 mg / L, respectively. When the p-TSA concentration of the substrate was much higher than that of 46.95mg/L, the degradation of p-TSA by strain Y-2 was in accordance with the zero-order kinetic model. That is, p-TSA degrades at maximum rate, independent of substrate concentration. When the concentration of substrate p-TSA is much lower than that of 46.95mg/L, the degradation rate of p-toluenesulfonic acid is first order reaction with the concentration of substrate, that is, the higher the substrate concentration, the faster the degradation rate of p-toluenesulfonic acid. At the intermediate concentration, the degradation of p-TSA by strain Y-2 was in the mixed reaction zone.
【學(xué)位授予單位】：南京農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：X172

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 曹華明;;極端微生物的研究及應(yīng)用[J];天津農(nóng)業(yè)科學(xué);2013年10期

2 魯亢;楊尚源;梁志偉;王云龍;李鑫;余華東;吳偉祥;;有機(jī)物生物降解性評價(jià)方法綜述[J];應(yīng)用生態(tài)學(xué)報(bào);2013年02期

3 崔靜嵐;陳晨;秦智慧;俞春娜;沈慧;沈超峰;陳英旭;;嗜熱菌對有機(jī)污染物的降解及其應(yīng)用研究進(jìn)展[J];應(yīng)用生態(tài)學(xué)報(bào);2012年11期

4 杜洪鳳;游釩;沈月華;汪良樞;;高效液相色譜法測定工作場所空氣中對甲苯磺酸[J];預(yù)防醫(yī)學(xué)情報(bào)雜志;2012年09期

5 曹侃;劉東飛;劉亞凱;翟志才;;3種芳香磺酸在超高交聯(lián)吸附樹脂上的吸附行為[J];水處理技術(shù);2012年04期

6 張凌;陶瑩;常志顯;李德亮;;臭氧法降解水中對甲基苯磺酸的動(dòng)力學(xué)研究[J];環(huán)境科學(xué)學(xué)報(bào);2011年10期

7 張明霞;焦光聯(lián);索超;;溫度對IMO-SBR中微生物特性的影響[J];工業(yè)用水與廢水;2011年04期

8 李青云;徐小芳;劉幽燕;周茂鐘;鐘善錦;;氯氰菊酯高效降解菌GF31的生長特性及其對污染土壤的修復(fù)能力[J];環(huán)境工程學(xué)報(bào);2011年06期

9 劉國生;郝曉潔;段佩玲;侯瑛;秦文艷;;苯酚降解菌UW7的鑒定及對苯酚的降解作用[J];應(yīng)用與環(huán)境生物學(xué)報(bào);2011年01期

10 劉勇;;苯磺酸稀溶液的絡(luò)合萃取研究[J];咸陽師范學(xué)院學(xué)報(bào);2010年06期

相關(guān)碩士學(xué)位論文 前5條

1 張奎;芳磺酸化合物絡(luò)合萃取研究[D];浙江大學(xué);2014年

2 王菲;施氏假單胞菌N2對含氧多環(huán)芳烴的生物降解特性研究[D];西安建筑科技大學(xué);2013年

3 趙靜;施氏假單胞菌N2降解酚類污染物的特性及機(jī)理研究[D];西安建筑科技大學(xué);2013年

4 張夢思;聯(lián)苯、吡啶降解菌的篩選及降解研究[D];武漢科技大學(xué);2011年

5 蘭亞紅;施氏假單胞桿菌的毒死蜱降解酶的基本特性及其制劑的研究[D];中國農(nóng)業(yè)科學(xué)院;2008年

，

本文編號：1778800