本文關(guān)鍵詞： 鄰苯二甲酸二丁酯 吸附 微生物 礦物 DMPP　出處：《湖南農(nóng)業(yè)大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：鄰苯二甲酸二丁酯(DBP)作為鄰苯二甲酸酯類(lèi)的一種,是環(huán)境中較為常見(jiàn)的環(huán)境激素類(lèi)有機(jī)污染物。本文從DBP染毒后的土壤污染樣品中篩選出一株對(duì)DBP有較高耐受性的微生物伯克霍爾德菌(Burkholderia),研究其對(duì)土壤中DBP的吸附行為和機(jī)制,及其與三種礦物(針鐵礦、高嶺土、蒙脫石)組成的復(fù)合體系對(duì)DBP的吸附行為和機(jī)制。在研究Burkholderia對(duì)DBP的吸附行為時(shí),從吸附動(dòng)力學(xué)、吸附等溫模型擬合、解吸等方面進(jìn)行分析,并考察了pH值、不同濃度的3,4-二甲基吡唑磷酸鹽(DMPP)等因素對(duì)菌體吸附DBP的影響,結(jié)合傅立葉轉(zhuǎn)換紅外光譜(FTIR)對(duì)Burkholderia吸附DBP前后的表面基團(tuán)的變化進(jìn)行了分析；在研究細(xì)菌-礦物復(fù)合體系吸附DBP時(shí),考察了復(fù)合體系的吸附量(率)和解吸量(率),及細(xì)菌礦物之間不同比例、不同DMPP濃度的影響。結(jié)合掃描電子顯微鏡(Scanning Electron Microscope, SEM)觀察Burkholderia與三種礦物復(fù)合后的表面形貌；同時(shí)通過(guò)三種復(fù)合體系疏水性實(shí)測(cè)值與預(yù)估值之間的對(duì)比,進(jìn)一步闡明了復(fù)合體系的吸附機(jī)理。通過(guò)以上大量實(shí)驗(yàn)初步探明了DBP在Burkholderia和三種礦物界面的吸附行為及機(jī)制。本文的研究結(jié)論有如下幾點(diǎn)：(1) Burkholderia吸附DBP的過(guò)程符合Freundlich吸附等溫模型,相關(guān)性R2=0.94,吸附等溫式為：qe=2A6×Ce1/1.02。(2)解吸實(shí)驗(yàn)發(fā)現(xiàn),Burkholderia對(duì)DBP的吸附有物理吸附和化學(xué)吸附,其中主要是化學(xué)吸附。(3)紅外光譜分析結(jié)果表明,細(xì)菌對(duì)DBP的吸附主要是通過(guò)C-H的伸縮振動(dòng)而相互作用在一起的。同時(shí),細(xì)菌表面的活性基團(tuán)和細(xì)胞壁也參與了吸附行為。(4)在1：1的混合比例下,復(fù)合體對(duì)DBP吸附的實(shí)測(cè)值與理論值之間的差異最大,對(duì)DBP吸附的影響較大。細(xì)菌-針鐵礦和細(xì)菌-蒙脫石的實(shí)際吸附量比理論值分別提高了1.10 mg/g和1.83 mg/g,而細(xì)菌-高嶺土則下降了1.16 mg/g。(5)通過(guò)掃描電鏡觀察發(fā)現(xiàn),微生物與三種礦物結(jié)合形態(tài)不同,與針鐵礦和蒙脫石結(jié)合緊密,與高嶺土結(jié)合松散。(6)不同濃度的DMPP加入對(duì)細(xì)菌的生長(zhǎng)、DBP的吸附和復(fù)合體系吸附DBP均有影響,且2 mmol/L的DMPP可以較大提高細(xì)菌-蒙脫石復(fù)合體系對(duì)DBP的吸附。
[Abstract]:Dibutyl phthalate (DBP) is a kind of phthalate ester. It is a common environmental hormone organic pollutant in the environment. In this paper, a microorganism with high tolerance to DBP was isolated from soil contaminated samples exposed to DBP. Burkholderia. The adsorption behavior and mechanism of DBP in soil and its relationship with three minerals (goethite and kaolin) were studied. The adsorption behavior and mechanism of DBP in montmorillonite (MMT) composite system. The adsorption kinetics and isotherm model of DBP adsorption by Burkholderia were fitted. The effects of pH value and different concentration of 3o 4- dimethylpyrazole phosphate on the adsorption of DBP were investigated. The changes of surface groups before and after Burkholderia adsorption on DBP were analyzed by Fourier transform infrared spectroscopy (FTIR). In the study of the adsorption of DBP by bacteria-mineral complex system, the adsorption capacity (rate) and desorption capacity (ratio) of the composite system were investigated, as well as the different ratios between bacteria and minerals. Combined with scanning electron microscope (SEM), scanning Electron Microscope. The surface morphology of Burkholderia combined with three minerals was observed. At the same time, the hydrophobicity of the three composite systems was compared with the estimated values. The adsorption mechanism of the composite system was further elucidated. The adsorption behavior and mechanism of DBP at the interface of Burkholderia and the three minerals were preliminarily proved by a large number of experiments mentioned above. The conclusion of this paper is as follows:. The following points:. (. 1) the process of DBP adsorption by Burkholderia accords with the isotherm model of Freundlich adsorption. The desorption experiment showed that the adsorption isotherm equation was: 1: QE 2A6 脳 ce 1 / 1.02.2.2. The adsorption of DBP by Burkholderia includes physical adsorption and chemisorption, mainly chemisorption. The adsorption of DBP by bacteria is mainly through C-H stretching vibration and interaction. At the same time. The active group and cell wall on the surface of bacteria also participated in the adsorption behavior. 4) under the mixed ratio of 1: 1, the difference between the measured values and the theoretical values of DBP adsorption by the complex was the largest. The adsorption capacity of bacteria-goethite and bacteria-montmorillonite was 1.10 mg/g and 1.83 mg/g higher than the theoretical values, respectively. On the other hand, bacteria-kaolin decreased by 1.16 mg / g 路L ~ (5). By scanning electron microscope, it was found that the microorganism binds closely with goethite and montmorillonite in different forms with the three minerals. The addition of different concentrations of DMPP to kaolinite could affect the adsorption of DBP on the growth of bacteria and the adsorption of DBP in the composite system. The adsorption of DBP on the bacteria-montmorillonite composite system could be greatly improved by 2 mmol/L DMPP.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：X592;X172

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