發(fā)布時(shí)間：2017-12-27 23:13

  本文關(guān)鍵詞：兩種碳源條件下好氧顆粒污泥中細(xì)菌的群落結(jié)構(gòu)研究　出處：《武漢理工大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 碳源 好氧顆粒污泥 菌群結(jié)構(gòu) 脫氮除磷

【摘要】：好氧顆粒污泥具有沉降性能優(yōu)異、微生物濃度較高等特點(diǎn),在污水脫氮除磷方面具有良好的應(yīng)用潛力。已有研究表明,碳源的組成對(duì)顆粒污泥的脫氮除磷性能具有重要影響,但目前關(guān)于碳源對(duì)顆粒污泥影響的研究主要集中在對(duì)污泥理化性質(zhì)和內(nèi)部結(jié)構(gòu)的影響方面,缺少對(duì)不同碳源條件下顆粒污泥中細(xì)菌,尤其是功能菌組成的系統(tǒng)研究。本文通過(guò)構(gòu)建16S rDNA文庫(kù),研究了分別以葡萄糖、黃水作為碳源的兩個(gè)序批式反應(yīng)器(SBR)中好氧顆粒污泥的菌群結(jié)構(gòu),并對(duì)不同碳源條件下顆粒污泥中細(xì)菌的多樣性和功能菌進(jìn)行了比較和分析,主要研究?jī)?nèi)容及結(jié)果如下:(1)分別構(gòu)建了兩種碳源條件下好氧顆粒污泥的16S rDNA文庫(kù),均得到155個(gè)陽(yáng)性克隆子。酶切分型結(jié)果表明,2個(gè)文庫(kù)可分別劃分為53個(gè)(葡萄糖文庫(kù))和90個(gè)(黃水文庫(kù))可分類(lèi)操作單元(OTU)。葡萄糖文庫(kù)的物種豐富度指數(shù)Schao1為89.2,黃水文庫(kù)的為183.2。黃水培養(yǎng)的顆粒污泥中細(xì)菌的多樣性遠(yuǎn)高于葡萄糖培養(yǎng)的顆粒污泥,說(shuō)明進(jìn)水基質(zhì)對(duì)顆粒污泥中細(xì)菌的多樣性有顯著影響。(2)對(duì)兩文庫(kù)中克隆子數(shù)大于1的OTU進(jìn)行了測(cè)序,分別獲得26條(葡萄糖文庫(kù))和30條(黃水文庫(kù))16S rDNA序列。序列分析結(jié)果表明,黃水文庫(kù)中已測(cè)序的細(xì)菌分別來(lái)自Proteobacteria、Acidobacteria、Chloroflexi和Bacteroidetes 4個(gè)門(mén),而葡萄糖文庫(kù)中除了這4個(gè)門(mén)的菌以外還檢測(cè)到了Candidatus Saccharibacteria門(mén)和Planctomycetes門(mén)的菌。(3)從屬的水平上講,2個(gè)文庫(kù)的菌群組成有很大的差異。葡萄糖文庫(kù)中只有一個(gè)能鑒定到屬的優(yōu)勢(shì)類(lèi)群(Candidatus Accumulibacter菌),該類(lèi)群占已測(cè)序克隆的23.4%;其它優(yōu)勢(shì)類(lèi)群均與已知菌屬同源性較低,它們均占已測(cè)序克隆數(shù)的5.5%~8.6%。黃水文庫(kù)的優(yōu)勢(shì)菌為T(mén)hauera、Dechloromonas、Nitrosomonas和Hydrogenophaga,分別占已測(cè)序克隆數(shù)的16.8%、8.4%、8.4%和5.3%;此外,還有少量與已知菌屬同源性較低的菌(占已測(cè)序克隆的5.3%~8.4%)。2個(gè)文庫(kù)優(yōu)勢(shì)菌的組成差異顯著,說(shuō)明碳源對(duì)顆粒污泥的菌群結(jié)構(gòu)有重要影響。(4)葡萄糖文庫(kù)和黃水文庫(kù)中均檢測(cè)到了與除磷和反硝化有關(guān)的細(xì)菌。2個(gè)文庫(kù)中的聚磷菌均為Candidatus Accumulibacter菌,但葡萄糖文庫(kù)中該菌所占的比例(23.4%)高于黃水文庫(kù)(8.4%)。葡萄糖文庫(kù)中只檢測(cè)到一種反硝化菌(Denitratisoma-like菌),而黃水文庫(kù)中與反硝化有關(guān)的菌包括Thauera、Dechloromonas、Zoogloea以及和1個(gè)與Sulfuritalea親緣關(guān)系較近的細(xì)菌類(lèi)群。此外,黃水文庫(kù)中反硝化菌在已測(cè)序克隆中所占的比例(29.5%)高于葡萄糖文庫(kù)(7.0%)。這些差異可能是由于2個(gè)反應(yīng)器N、P負(fù)荷不同引起的。(5)黃水文庫(kù)中檢測(cè)到了可能具有降解芳香族化合物功能、氨氧化功能或能夠分泌胞外多聚物(EPS)的菌,而在葡萄糖文庫(kù)中沒(méi)有檢測(cè)到具有這些功能的菌,說(shuō)明黃水培養(yǎng)的顆粒污泥細(xì)菌的功能多樣性更豐富,更適合用來(lái)處理成分復(fù)雜的有機(jī)廢水。
[Abstract]:Aerobic granular sludge has the characteristics of excellent settling property and high microbial concentration, and has good application potential in the aspect of nitrogen and phosphorus removal in sewage. Studies have shown that the composition of carbon source has important influence on the performance of nitrogen and phosphorus removal of granular sludge, but the current research on the effects of carbon source on the granular sludge mainly concentrated on the influence of physicochemical properties and internal structure of sludge, lack of bacteria in granular sludge under different carbon source conditions, especially the system of functional bacteria. In this paper, by constructing the 16S rDNA library, was studied by glucose, water was used as carbon source two sequencing batch reactor (SBR) flora in aerobic granular sludge, and the diversity and function of bacteria granular sludge with different carbon source conditions were compared and analyzed. The main research contents and the results are as follows: (1) were constructed with 16S rDNA Library of aerobic granular sludge under the condition of two kinds of carbon sources, obtained 155 positive clones. RFLP results show that 2 libraries can be divided into 53 (glucose Library) and 90 (yellow Library) classification operation unit (OTU). The species richness index of Schao1 glucose was up to 89.2, water was up to 183.2. The water culture of the diversity of bacteria in granular sludge is much higher than that of granular sludge on glucose, and that water matrix on bacterial diversity in granular sludge had significant effect. (2) of two library clones number is greater than 1 OTU were sequenced, respectively (26 glucose Library) and 30 (yellow Library) 16S rDNA sequence. The results of sequence analysis showed that the bacteria in the water have been sequencing library respectively from Proteobacteria, Acidobacteria, Chloroflexi and Bacteroidetes 4, while glucose library besides these 4 door bacteria were detected Candidatus Saccharibacteria and Planctomycetes gates of bacteria. (3) from the level of the subordinate, there is a great difference in the composition of the bacteria in the 2 library. Only one of the glucose libraries can identify the dominant group (Candidatus Accumulibacter), which accounts for 23.4% of the cloned clones, and the other dominant groups are low homology with the known bacteria. They all account for 5.5%~8.6% of the number of sequenced clones. The dominant bacteria yellow library was Thauera and Dechloromonas, Nitrosomonas and Hydrogenophaga, respectively, accounted for 16.8% of the number of sequenced clones, 8.4%, 8.4% and 5.3%; in addition, there are a small number of known bacteria with low homology bacteria (representing sequencing cloned 5.3%~8.4%). There were significant differences in the composition of the dominant bacteria in the 2 libraries, indicating that the carbon source had an important influence on the structure of the bacterial flora of the granular sludge. (4) glucose library and library water were detected with phosphorus removal and denitrification of bacteria. PAOs 2 libraries were Candidatus Accumulibacter bacteria, but bacteria accounted for the proportion of the glucose Library (23.4%) is higher than that of yellow Library (8.4%). Glucose library only detected a denitrifying bacteria (Denitratisoma-like bacteria), and yellow library and denitrifying bacteria including related bacterial taxa near Thauera, Dechloromonas, Zoogloea, and 1 Sulfuritalea and genetic relationship. In addition, denitrifying bacteria yellow library occupied in the sequenced clones in the library (29.5%) is higher than the proportion of glucose (7%). These differences may be caused by the different loads of the 2 reactors, N and P. (5) was detected in the library may have aromatic compound degrading function, ammonia oxidation function or secretion of extracellular polymeric substances (EPS) of the bacteria, but not in the glucose library were detected with these features that water cultured bacteria, granular sludge bacterial functional diversity is more abundant, more suitable for the treatment of organic wastewater by complex components.
【學(xué)位授予單位】：武漢理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：X703;X172

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