光合細(xì)菌協(xié)同產(chǎn)氣腸桿菌聯(lián)合發(fā)酵制氫試驗
發(fā)布時間：2018-11-05 07:40
【摘要】：暗-光聯(lián)合生物制氫是提高底物利用率和產(chǎn)氫潛力的有益探索。該文以玉米秸稈酶解液為產(chǎn)氫底物,采用光合細(xì)菌(HAU-M1)與產(chǎn)氣腸桿菌(AS1.489)混合培養(yǎng)工藝,進(jìn)行了同步糖化暗-光聯(lián)合生物制氫試驗研究。以累積產(chǎn)氫量為主要指標(biāo),利用單因素試驗考察了底物質(zhì)量濃度、初始pH值、光照強(qiáng)度、發(fā)酵溫度對HAU-M1與產(chǎn)氣腸桿菌混合培養(yǎng)條件下聯(lián)合產(chǎn)氫的影響,并在單因素試驗的基礎(chǔ)上通過正交試驗對產(chǎn)氫工藝參數(shù)進(jìn)行了優(yōu)化。結(jié)果表明:各工藝參數(shù)對HAU-M1與產(chǎn)氣腸桿菌聯(lián)合產(chǎn)氫影響的主次順序為:發(fā)酵溫度初始pH值底物質(zhì)量濃度光照強(qiáng)度。發(fā)酵溫度和初始pH值是影響HAU-M1與產(chǎn)氣腸桿菌聯(lián)合產(chǎn)氫的顯著因素。HAU-M1與產(chǎn)氣腸桿菌混合培養(yǎng)聯(lián)合產(chǎn)氫的較佳工藝條件為:底物質(zhì)量濃度35 g/L、初始pH值6.5、光照強(qiáng)度3 500 1x、發(fā)酵溫度30℃,在此條件下,72 h的累積產(chǎn)氫量達(dá)到332.6 mL,單位產(chǎn)氫量為47.5 mL/g。該試驗研究可為基于秸稈類生物質(zhì)的暗-光細(xì)菌混合培養(yǎng)聯(lián)合產(chǎn)氫的進(jìn)一步研究提供參考。
[Abstract]:Dark-light combined biological hydrogen production is a useful exploration to improve substrate utilization and hydrogen production potential. In this paper, the enzymatic hydrolysis solution of corn straw was used as the substrate for hydrogen production, and the mixed culture technology of photosynthetic bacteria (HAU-M1) and Enterobacter aerogenes (AS1.489) was used to produce hydrogen by simultaneous saccharification of dark light and light. The effects of substrate mass concentration, initial pH value, light intensity and fermentation temperature on hydrogen production under mixed culture conditions of HAU-M1 and Enterobacter aerogenes were investigated by single factor experiment. On the basis of single factor test, the process parameters of hydrogen production were optimized by orthogonal test. The results showed that the primary and secondary order of the effects of various technological parameters on the hydrogen production of HAU-M1 and Enterobacter aerogenes was as follows: the initial substrate concentration of pH at fermentation temperature and light intensity. Fermentation temperature and initial pH value were significant factors affecting hydrogen production by HAU-M1 and Enterobacter aerogenes. The optimum conditions for hydrogen production by mixed culture of HAU-M1 and Enterobacter aerogenes were as follows: mass concentration of substrate 35 g / L, initial pH 6.5; Under the conditions of light intensity 3 5001x, fermentation temperature 30 鈩，
本文編號：2311372
[Abstract]:Dark-light combined biological hydrogen production is a useful exploration to improve substrate utilization and hydrogen production potential. In this paper, the enzymatic hydrolysis solution of corn straw was used as the substrate for hydrogen production, and the mixed culture technology of photosynthetic bacteria (HAU-M1) and Enterobacter aerogenes (AS1.489) was used to produce hydrogen by simultaneous saccharification of dark light and light. The effects of substrate mass concentration, initial pH value, light intensity and fermentation temperature on hydrogen production under mixed culture conditions of HAU-M1 and Enterobacter aerogenes were investigated by single factor experiment. On the basis of single factor test, the process parameters of hydrogen production were optimized by orthogonal test. The results showed that the primary and secondary order of the effects of various technological parameters on the hydrogen production of HAU-M1 and Enterobacter aerogenes was as follows: the initial substrate concentration of pH at fermentation temperature and light intensity. Fermentation temperature and initial pH value were significant factors affecting hydrogen production by HAU-M1 and Enterobacter aerogenes. The optimum conditions for hydrogen production by mixed culture of HAU-M1 and Enterobacter aerogenes were as follows: mass concentration of substrate 35 g / L, initial pH 6.5; Under the conditions of light intensity 3 5001x, fermentation temperature 30 鈩，
本文編號：2311372
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