本文選題：碳量子點　切入點：色譜分離　出處：《東北師范大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：碳量子點(CQDs)作為一類新型熒光納米材料,近年來受到了廣泛的關(guān)注和研究。其無毒無環(huán)境污染、生物相容性好、易于功能化,且制備方法簡單多樣、碳源豐富而且價廉,因此應(yīng)用領(lǐng)域十分寬廣。本文在前人工作的基礎(chǔ)上,對碳量子點的合成、分離與提純、應(yīng)用等做了進(jìn)一步的研究和探索。主要內(nèi)容如下:1.以殼聚糖為原料,酸性條件下通過水熱法合成了可產(chǎn)生藍(lán)色熒光的碳量子點。用熒光光譜、核磁共振波譜、質(zhì)譜、紫外-可見吸收光譜、透射電鏡、紅外光譜等多種先進(jìn)檢測手段對其結(jié)構(gòu)、形貌以及性質(zhì)進(jìn)行了表征,并根據(jù)質(zhì)譜,對其形成機(jī)理和可能的結(jié)構(gòu)進(jìn)行了推測。通過細(xì)胞溶血實驗,對所制備的碳量子點的生物相容性進(jìn)行了評估。2.利用高效液相色譜對碳量子點進(jìn)行分離提純,通過改變固定相、流動相及流速等色譜條件,對碳量子點溶液進(jìn)行了有效的制備分離,結(jié)合質(zhì)譜分析,進(jìn)一步推斷了制備分離出的碳量子點的可能結(jié)構(gòu),測定了熒光量子產(chǎn)率。3.將用苯丙氨酸修飾后的碳量子點分別與鄰、對硝基苯酚異構(gòu)體作用,通過考察二異構(gòu)體對碳量子點熒光強(qiáng)度的影響,探討了碳量子點應(yīng)用于硝基苯酚異構(gòu)體識別的最佳條件。本文的研究表明,利用高效液相色譜分離純化碳量子點,可以極大地提高碳量子點的熒光量子效率,為解決碳量子點組成復(fù)雜、熒光共振明顯從而導(dǎo)致熒光量子效率低下并制約碳量子點應(yīng)用前景的瓶頸問題,進(jìn)行了有益的嘗試。將碳量子點應(yīng)用于異構(gòu)體的熒光識別,對開拓碳量子點的應(yīng)用領(lǐng)域具有積極的作用。
[Abstract]:As a new kind of fluorescent nanomaterials, carbon quantum dots (CQDs) have been widely studied in recent years. They are non-toxic and non-polluting, have good biocompatibility, are easy to be functionalized, and can be prepared by simple and diverse methods, rich in carbon sources and cheap in price. In this paper, the synthesis, separation, purification and application of carbon quantum dots are further studied and explored. The main contents are as follows: 1. Using chitosan as raw material. Carbon quantum dots, which can produce blue fluorescence, were synthesized by hydrothermal method under acidic conditions. Their structures were characterized by fluorescence spectra, NMR spectra, mass spectra, UV-Vis absorption spectra, transmission electron microscopy, infrared spectra, etc. The morphology and properties were characterized, and its formation mechanism and possible structure were inferred by mass spectrometry. The biocompatibility of the prepared carbon quantum dots was evaluated. 2. The separation and purification of carbon quantum dots were carried out by high performance liquid chromatography (HPLC), and the chromatographic conditions such as stationary phase, mobile phase and flow rate were changed. The effective preparation and separation of carbon quantum dot solution were carried out. The possible structure of the carbon quantum dot was further inferred by mass spectrometry, and the fluorescence quantum yield was determined. 3. The carbon quantum dot modified with phenylalanine was separated from adjacent carbon quantum dot. The effects of diisomers on the fluorescence intensity of carbon quantum dots (QDs) were investigated, and the optimum conditions for the application of QDs to the recognition of nitrophenol isomers were discussed. The fluorescence quantum efficiency of carbon quantum dots can be greatly improved by the separation and purification of carbon quantum dots by high performance liquid chromatography. The fluorescence resonance obviously leads to the low fluorescence quantum efficiency and restricts the application prospect of carbon quantum dots, and a useful attempt has been made to apply carbon quantum dots to the fluorescence recognition of isomers. It plays an active role in developing the application field of carbon quantum dots.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TQ127.11;O657

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