本文選題：微生物學(xué) + 裂褶菌��； 參考：《中國(guó)科技論文》2016年18期

【摘要】：采用平板和液體培養(yǎng)技術(shù),研究了1株海洋源裂褶菌(Schizophyllum commune MCCC 3A00233)降解多環(huán)芳烴(polycyclic aromatic hydrocarbons,PAHs)類化合物菲、熒蒽的效率及機(jī)理。結(jié)果表明:裂褶菌MCCC 3A00233對(duì)菲、熒蒽具有較高的耐受性,在質(zhì)量濃度高達(dá)200mg/L的平板上,菌絲生長(zhǎng)的抑制率≤25%;當(dāng)菲、熒蒽的質(zhì)量濃度為50mg/L時(shí),菌株生長(zhǎng)最快,培養(yǎng)10d對(duì)菲、熒蒽的降解率≥80%,降解產(chǎn)物對(duì)植物、微生物的毒性大大減小。另外,該菌對(duì)菲、熒蒽的降解作用主要是通過(guò)產(chǎn)生錳過(guò)氧化物酶(manganese peroxidase,MnP),其次為木質(zhì)素過(guò)氧化物酶(lignin peroxidase,LiP)和漆酶(Laccase)而起作用的,其中Lip和Laccase在該菌降解菲、熒蒽的起始階段發(fā)揮重要作用。這些結(jié)果表明,裂褶菌MCCC 3A00233是1株高效降解菲、熒蒽的白腐真菌,可用于海水中PAHs類污染物的降解。
[Abstract]:The efficiency and mechanism of degradation of phenanthrene (phenanthrene) and fluoranthene (fluoranthene) by a strain of Schizophyllum commune MCCC 3A00233) were studied by plate and liquid culture techniques. The results showed that MCCC 3A00233 had higher tolerance to phenanthrene and fluoranthene, and the inhibition rate of hyphal growth was 鈮，

本文編號(hào)：1908224