本文選題：拉曼 + 熒光��； 參考：《光譜學與光譜分析》2017年10期

【摘要】：深海熱液環(huán)境中存在著巨大的化學和熱梯度,快速劇烈的混合和生物過程產(chǎn)生了多種多樣的礦物過程,并培養(yǎng)了大量的化學合成微生物。激光拉曼光譜非常適合于深海熱液環(huán)境礦物過程的探測,然而要對礦物與微生物作用過程進行研究,還需要與熒光光譜技術(shù)進行聯(lián)合,針對此需求開展了原理驗證實驗研究。在實驗室搭建了一套拉曼-熒光聯(lián)合光譜探測桌面系統(tǒng),利用一臺雙波長激光器同時作為拉曼光譜和熒光光譜的激發(fā)光源,其中拉曼光譜采用532nm波長,熒光光譜采用266nm波長,雙波長激光器發(fā)出的光束經(jīng)分光鏡分為兩路,經(jīng)過后向散射光路收集的兩路信號分別進入兩個小型光纖光譜儀進行分光探測,拉曼光譜采用QE65000光譜儀,熒光光譜采用USB2000光譜儀,通過軟件可以方便的分別設(shè)置兩個光譜儀的參數(shù)。利用搭建的實驗系統(tǒng)對海水和擬菱形藻樣品進行探測,分別獲得了海水樣品的SO2-4拉曼光譜和可溶性有機物(CDOM)熒光光譜,擬菱形藻樣品的類胡蘿卜素拉曼光譜和類蛋白、葉綠素等熒光光譜,實驗結(jié)果證明了研制小型拉曼-熒光聯(lián)合光譜探測裝置的可行性,并為發(fā)展原位聯(lián)合光譜探測裝置提供了技術(shù)參考。
[Abstract]:There are huge chemical and thermal gradients in deep-sea hydrothermal environment. The rapid and violent mixing and biological processes produce a variety of mineral processes and a large number of chemosynthetic microorganisms have been cultured. Laser Raman spectroscopy is very suitable for the detection of mineral processes in deep-sea hydrothermal environment. However, in order to study the interaction between minerals and microorganisms, it is necessary to combine with fluorescence spectroscopy. A set of Raman fluorescence detection desktop system was set up in the laboratory. A dual wavelength laser was used as the excitation source of Raman spectrum and fluorescence spectrum. The Raman spectrum used 532nm wavelength and the fluorescence spectrum used 266nm wavelength. The beams from the dual-wavelength laser are divided into two channels through the spectroscope, and the two signals collected through the backscattering light path enter into two small optical fiber spectrometers respectively for spectroscopic detection. The Raman spectrum is QE65000 spectrometer, and the fluorescence spectrum is USB2000 spectrometer. The parameters of the two spectrometers can be easily set by software. The SO2-4 Raman spectra and soluble organic compounds fluorescence spectra, carotenoid Raman spectra and protein-like spectra of sea water samples were obtained by using the experimental system to detect sea water and diamond-like alga, respectively, and the results were as follows: (1) the SO2-4 Raman spectra of sea water samples and the fluorescence spectra of soluble organic compounds were obtained, and the carotenoid Raman spectra and protein like spectra were obtained. The experimental results show that it is feasible to develop a small Raman fluorescence combined spectrometer and provide a technical reference for the development of in situ joint spectroscopic detector.
【作者單位】： 中國海洋大學信息科學與工程學院;
【基金】：國家自然科學基金項目(61575181) 國家(863計劃)項目(2012AA09A405)資助
【分類號】：O657.3;P714

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