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幾種新型固態(tài)抗菌材料的制備

發(fā)布時(shí)間:2019-05-23 20:22
【摘要】:微生物存在于生活境中的各個(gè)角落,以這樣或那樣的方式影響著我們的生活,他們之中有的是維持生態(tài)環(huán)境不可或缺的重要一環(huán),有的則對(duì)人們的生命健康造成了嚴(yán)重的威脅,為了生存,人們不得不用盡各種方法去抵抗微生物對(duì)健康的侵蝕,抗生素是人們抵御病原微生物的重要武器,但細(xì)菌的耐藥性已然成為一個(gè)日趨嚴(yán)峻的問(wèn)題,新藥開(kāi)發(fā)的速度遠(yuǎn)遠(yuǎn)趕不上細(xì)菌產(chǎn)生耐藥性的速度,要解決這一問(wèn)題除了合理規(guī)范用藥,堅(jiān)持健康的生活習(xí)慣,就要從根本上減少人與病原微生物接觸的機(jī)會(huì)-廉價(jià)高效的消毒劑的大范圍使用是必然的,鑒于大部分消毒劑的強(qiáng)刺激性,在一些不適宜使用消毒劑的場(chǎng)合,在諸如公共健身休閑娛樂(lè)器材的表面使用溫和、持久高效的高分子抗菌涂層就顯得十分必要。本文嘗試在兩種不同的基質(zhì)上枝接高分子季銨鹽和多粘菌素,一種基質(zhì)是以廢棄的啤酒酵母為原料,通過(guò)水熱反應(yīng)得到的常溫下十分穩(wěn)定的兩親性酵母碳渣球,另一基質(zhì)為通過(guò)水熱反應(yīng)合成的納米四氧化三鐵微球,在此基礎(chǔ)上均以蒸餾沉淀法進(jìn)一步枝接聚4-乙烯基吡啶,在混合溶劑中使用兩種不同的鹵化物與聚4-乙烯基吡啶成鹽;以EDC、NHS作為活化劑將多粘菌素修飾在碳渣微球的表面,使用馬爾文激光粒度儀,傅立葉紅外光譜儀,掃描與透射電子顯微鏡,熱重分析儀等分析檢測(cè)儀器對(duì)修飾前后的碳渣微球、四氧化三鐵微球的化學(xué)組成和微觀形態(tài)進(jìn)行檢測(cè)分析。利用高效液相對(duì)碳渣微球表面的多粘菌素進(jìn)行含量的測(cè)定,使用紫外分光光度法測(cè)定聚4-乙烯基吡啶修飾的微球表面的電荷數(shù)。以大腸桿菌和金黃色葡萄球菌為實(shí)驗(yàn)菌種,以蒸餾水為分散介質(zhì)模擬自然水環(huán)境,對(duì)材料的抗菌活性進(jìn)行測(cè)定。
[Abstract]:Microbes exist in every corner of life and affect our lives in one way or another. Some of them are indispensable to maintaining the ecological environment, while others pose a serious threat to people's lives and health. In order to survive, people have to use all kinds of methods to resist the erosion of health by microorganisms. Antibiotics are an important weapon for people to resist pathogenic microorganisms, but the drug resistance of bacteria has become an increasingly serious problem. The speed of new drug development is far from keeping pace with the drug resistance of bacteria. In addition to reasonably standardizing the use of drugs and adhering to healthy living habits, it is necessary to solve this problem. It is necessary to fundamentally reduce the chance of human contact with pathogenic microorganisms-the wide use of cheap and efficient disinfectants is inevitable, in view of the strong irritation of most disinfectants, in some cases where disinfectants are not suitable for use, It is necessary to use mild, lasting and efficient polymer antibacterial coating on the surface of public fitness leisure and entertainment equipment. In this paper, polymer quaternary ammonium salt and polymyxin were seeded on two different medium. one kind of matrix was a very stable Amphiphilic yeast carbon slag ball obtained by hydrothermal reaction using waste beer yeast as raw material. The other matrix is nanometer iron tetroxide microspheres synthesized by hydrothermal reaction, on the basis of which 4-vinylpyridine is further grafting by distillation precipitation method. Two different halides were used in the mixed solvent to form salt with poly4-vinylpyridine. Polymyxin was modified on the surface of carbon slag microspheres with EDC,NHS as activating agent. Ma Erwen laser particle size meter, Fourier transform infrared spectrometer, scanning and transmission electron microscope were used. The chemical composition and micromorphology of carbon slag microspheres and iron tetroxide microspheres before and after modification were detected and analyzed by thermogravimetric analyzer and other instruments. The content of polymyxin on the surface of carbon slag microspheres was determined by high efficiency liquid, and the charge number on the surface of poly4-vinylpyridine modified microspheres was determined by ultraviolet spectrophotometry. Escherichia coli and Staphylococcus aureus were used as experimental strains and distilled water as dispersion medium to simulate the natural water environment, and the antibacterial activity of the materials was determined.
【學(xué)位授予單位】:河南大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2015
【分類號(hào)】:TB34

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