熒光性的金屬納米簇合成及傳感性能研究
發(fā)布時(shí)間：2018-11-18 18:10
【摘要】：近些年來(lái),熒光標(biāo)記技術(shù)已經(jīng)深入到人類研究的各個(gè)方面,如生物醫(yī)藥領(lǐng)域等。熒光標(biāo)記材料分為有機(jī)染料、半導(dǎo)體量子點(diǎn)、復(fù)合熒光納米粒子以及貴金屬納米簇,由于后三種熒光材料的粒子尺寸都在納米級(jí)別上,我們又將其合稱為熒光納米標(biāo)記材料。因其具有優(yōu)良的光化學(xué)性質(zhì),贏得了廣大研究者的關(guān)注,并成為新一代的熒光標(biāo)記材料。我們所說(shuō)的金屬納米簇,是指由Au、Ag或Cu等金屬的幾個(gè)至幾十個(gè)原子組成的一類新型的熒光納米材料。它們的粒子尺寸一般小于2nm,因其優(yōu)異的光譜和光物理性質(zhì)而受到科學(xué)家們的青睞。其中,迄今為止,利用脫氧核糖核酸(DNA)為基本骨架,合成的貴金屬納米簇已廣泛應(yīng)用于熒光成像、生物化學(xué)傳感、納米器件、抗菌劑、環(huán)境監(jiān)測(cè)等諸多領(lǐng)域。本論文則利用不同的DNA模板合成Ag NCs,實(shí)現(xiàn)對(duì)DNA序列的選擇性識(shí)別,并利用熒光銀納米簇為探針檢測(cè)Hg2+,同時(shí)對(duì)熒光銅納米粒子進(jìn)行了初步的研究。本文的主要研究?jī)?nèi)容和實(shí)驗(yàn)結(jié)論如下：1.采用(CNG)n序列DNA為模板,以NaBH4為還原劑合成具有熒光性質(zhì)的銀納米簇,這是一種快速、高效、傳統(tǒng)的合成銀納米簇的方法。通過(guò)紫外可見(jiàn)光譜和熒光光譜等表征方法研究了此類銀納米簇(Ag NCs)的熒光性質(zhì),并通過(guò)有機(jī)分子擁擠條件進(jìn)一步提高了以DNA為模板合成的熒光性銀納米簇的選擇性和靈敏度,為檢測(cè)(CGG)序列提供了一種新的可能性。實(shí)驗(yàn)結(jié)果表明：(CNG)n序列為模板合成的銀納米簇的熒光發(fā)射對(duì)DNA序列選擇依賴性的順序?yàn)镚CT≈A,這種選擇性順序在不同長(zhǎng)度(CNG)n序列上依然適用。此外,此方法優(yōu)越性在于利用有機(jī)分子提高了Ag NCs選擇性識(shí)別(CGG)的靈敏度,進(jìn)一步說(shuō)明了該實(shí)驗(yàn)方法的高效、便捷以及合理性。2. 主要以Three-way DNA為模板,以NaBH4為還原劑合成熒光銀納米簇,優(yōu)化了反應(yīng)物摩爾比、反應(yīng)時(shí)間等合成條件。通過(guò)熒光光譜等手段研究了該類型銀納米簇的熒光性質(zhì),并簡(jiǎn)單介紹了DNA/Ag NCs在檢測(cè)汞離子中的應(yīng)用。實(shí)驗(yàn)結(jié)果表明：含有胞嘧啶堿基的Three-way DNA能更有效地合成Ag NCs,這進(jìn)一步說(shuō)明銀納米簇的合成強(qiáng)烈依賴于DNA序列,而且合成的納米簇還可以用來(lái)檢測(cè)Hg2+離子。3. 以一些均聚物即含多個(gè)相同堿基組成的單鏈DNA為模板,選用抗壞血酸鈉作還原劑來(lái)合成熒光性的銅納米粒子,利用熒光穩(wěn)態(tài)的方法研究了DNA堿基對(duì)銅納米粒子生長(zhǎng)的選擇性以及穩(wěn)定性的影響。實(shí)驗(yàn)結(jié)果表明：含有多個(gè)胸腺嘧啶堿基的ss-DNA模板能夠有效的合成熒光性質(zhì)的銅納米粒子,且其熒光強(qiáng)度隨著堿基長(zhǎng)度的增加而增加。
[Abstract]:In recent years, fluorescent labeling techniques have been applied to all aspects of human research, such as biomedicine. Fluorescent labeling materials are divided into organic dyes semiconductor quantum dots composite fluorescent nanoparticles and noble metal nanoclusters. Because the size of the latter three fluorescent materials are at the nanometer level we call them fluorescent nanolabelling materials. Because of its excellent photochemical properties, it has attracted the attention of researchers and become a new generation of fluorescent labeling materials. The metal nanoclusters are a new kind of fluorescent nanomaterials composed of several to dozens of atoms of metals such as Au,Ag or Cu. They are generally smaller than 2 nm in size and are favored by scientists for their excellent spectral and photophysical properties. Up to now, the synthetic noble metal nanoclusters have been widely used in many fields, such as fluorescence imaging, biochemical sensing, nano-devices, antimicrobial agents, environmental monitoring and so on, using deoxyribonucleic acid (DNA) as the basic skeleton. In this thesis, Ag NCs, was synthesized by different DNA templates to realize the selective recognition of DNA sequences, and the fluorescent silver nanoclusters were used as probes to detect Hg2, and the fluorescent copper nanoparticles were studied preliminarily. The main research contents and experimental results are as follows: 1. Silver nanoclusters with fluorescence properties were synthesized by using (CNG) n sequence DNA as template and NaBH4 as reducing agent. This is a fast, efficient and traditional method for the synthesis of silver nanoclusters. The fluorescence properties of silver nanoclusters (Ag NCs) were studied by UV-Vis and fluorescence spectra. The selectivity and sensitivity of fluorescent silver nanoclusters synthesized using DNA as template were further improved under the condition of organic molecular crowding, which provided a new possibility for the detection of (CGG) sequences. The experimental results show that the order of fluorescence emission dependence on DNA sequence of silver nanoclusters synthesized by: (CNG) n sequence is GCT 鈮，
本文編號(hào)：2340756
[Abstract]:In recent years, fluorescent labeling techniques have been applied to all aspects of human research, such as biomedicine. Fluorescent labeling materials are divided into organic dyes semiconductor quantum dots composite fluorescent nanoparticles and noble metal nanoclusters. Because the size of the latter three fluorescent materials are at the nanometer level we call them fluorescent nanolabelling materials. Because of its excellent photochemical properties, it has attracted the attention of researchers and become a new generation of fluorescent labeling materials. The metal nanoclusters are a new kind of fluorescent nanomaterials composed of several to dozens of atoms of metals such as Au,Ag or Cu. They are generally smaller than 2 nm in size and are favored by scientists for their excellent spectral and photophysical properties. Up to now, the synthetic noble metal nanoclusters have been widely used in many fields, such as fluorescence imaging, biochemical sensing, nano-devices, antimicrobial agents, environmental monitoring and so on, using deoxyribonucleic acid (DNA) as the basic skeleton. In this thesis, Ag NCs, was synthesized by different DNA templates to realize the selective recognition of DNA sequences, and the fluorescent silver nanoclusters were used as probes to detect Hg2, and the fluorescent copper nanoparticles were studied preliminarily. The main research contents and experimental results are as follows: 1. Silver nanoclusters with fluorescence properties were synthesized by using (CNG) n sequence DNA as template and NaBH4 as reducing agent. This is a fast, efficient and traditional method for the synthesis of silver nanoclusters. The fluorescence properties of silver nanoclusters (Ag NCs) were studied by UV-Vis and fluorescence spectra. The selectivity and sensitivity of fluorescent silver nanoclusters synthesized using DNA as template were further improved under the condition of organic molecular crowding, which provided a new possibility for the detection of (CGG) sequences. The experimental results show that the order of fluorescence emission dependence on DNA sequence of silver nanoclusters synthesized by: (CNG) n sequence is GCT 鈮，
本文編號(hào)：2340756
本文鏈接：http://www.sikaile.net/kejilunwen/cailiaohuaxuelunwen/2340756.html
最近更新
教材專著
