【摘要】：以靜電吸附力將多聚賴氨酸(PLL)自組裝于玻璃基底上,制備了荷正電的PLL膜。研究了溶液p H、自組裝時間、濃度等對成膜質(zhì)量和吸附細菌性能的影響。研究表明,p H=7.4、時間為45 min、濃度為0.200 mg/m L為最佳組裝條件。以大腸桿菌和金黃色葡萄球菌作為供試菌,通過涂板計數(shù)和顯微鏡計數(shù)法測試了荷正電膜對細菌的吸附能力。結果表明,在最佳條件下制備的PLL膜在30 min內(nèi)對大腸桿菌吸附率達到92.3%,在45 min內(nèi)對金黃色葡萄球菌的吸附率達到83.0%,其對革蘭陰性菌的吸附率略大于對革蘭陽性菌的吸附率。
[Abstract]:Poly (lysine) (PLL) was self-assembled on glass substrate by electrostatic adsorption and positively charged PLL films were prepared. The effects of solution pH, self-assembly time and concentration on the quality of film formation and the adsorption of bacteria were studied. The results show that the best assembly condition is 7.4HH and 0.200 mg/m / L for 45 min,. Using Escherichia coli and Staphylococcus aureus as test bacteria, the adsorption ability of positive charge membrane to bacteria was tested by plate counting and microscope counting. The results showed that the adsorption rate of PLL membrane to Escherichia coli reached 92.3 in 30 min and 83.0 to Staphylococcus aureus in 45 min. The adsorption rate of Gram-negative bacteria was slightly higher than that of Gram-positive bacteria.
【作者單位】： 南京工業(yè)大學化學與分子工程學院;
【基金】：國家自然科學基金項目(21176124)
【分類號】：TB383.2
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本文編號：2257684