本文選題：納米粒 + 葉酸��； 參考：《中國組織工程研究》2017年14期

【摘要】：背景:研究發(fā)現(xiàn),納米材料與細胞接觸時可誘導(dǎo)細胞產(chǎn)生氧化應(yīng)激反應(yīng)、過敏反應(yīng),進而產(chǎn)生細胞毒性和基因毒性,因此,對納米毒理學的研究越來越受到廣泛的關(guān)注。目的:比較Pluronic(P85,F127和F87)三嵌段共聚物經(jīng)葉酸(folic acid,FA)和聚乳酸[poly(lactic acid),PLA]修飾前后的納米結(jié)合物的細胞相容性。方法:對Pluronic三嵌段共聚物(P85,F127和F87)進行修飾,在其兩端接上聚乳酸和葉酸,分別合成了多種兩親性嵌段共聚物(PLA-P85-PLA,FA-P85-PLA,PLA-F127-PLA,FA-F127-PLA,PLA-F87-PLA和FA-F87-PLA)納米粒子。選取HepG-2細胞為研究對象,從細胞形態(tài)、細胞代謝活性、細胞膜效應(yīng)來評價納米粒子的細胞毒性。結(jié)果與結(jié)論:(1)未經(jīng)葉酸和聚乳酸修飾的P85,F127和F87納米粒子(5,10,20,50,100 mg/L)作用Hep G-2細胞24 h后,與對照組相比,細胞相對增長率差異無顯著性意義(P0.05),且在低質(zhì)量濃度范圍內(nèi)(5,10,20,50 mg/L)有促進細胞增殖的作用;當質(zhì)量濃度增加到400 mg/L時,P85和F87納米粒子對細胞增殖有明顯的抑制作用;(2)經(jīng)葉酸和聚乳酸修飾的P85,F127和F87納米粒子(5,10,20,50,100,200,400 mg/L)作用Hep G-2細胞后,與對照組相比,差異無顯著性意義(P0.05);(3)結(jié)果表明,用葉酸和聚乳酸修飾Pluronic(P85,F127和F87)三嵌段共聚物,可提高其細胞相容性,合成的PLA-Pluronic-PLA納米粒子和FA-Pluronic-PLA納米粒子可用作藥物載體。
[Abstract]:Background: it has been found that nanomaterials contact with cells can induce oxidative stress reaction, allergic reaction, and then produce cytotoxicity and genetic toxicity. Therefore, the study of nano-toxicology has attracted more and more attention. Aim: to compare the cytocompatibility of pluronic (P85F127 and F87) triblock copolymers modified with folic acido FA and polylactic acid (poly (lactic acid). Methods: Pluronic triblock copolymers (P85 F127 and F87) were modified with polylactic acid and folic acid at the ends of the copolymers. A variety of amphiphilic block copolymers (PLA-P85-PLAFA-P85-PLAP-F127-APLFA-F127-PLA-F87-PLA and FA-F87-PLA) nanoparticles were synthesized. HepG-2 cells were selected to evaluate the cytotoxicity of nanoparticles in terms of cell morphology, cell metabolic activity and cell membrane effect. Results and conclusion: (1) Hep G-2 cells were treated with P85F127 and F87 nanoparticles without folic acid and polylactic acid for 24 h, compared with control group. There was no significant difference in cell relative growth rate (P0.05), and in the range of low mass concentration (510g / L), the cell proliferation was promoted. The proliferation of Hep G-2 cells was significantly inhibited by P85 and F87 nanoparticles modified by folic acid and polylactic acid (P85 / F127 and F87 nanoparticles modified by folic acid and polylactic acid) at a concentration of 400 mg / L, (2) Hep G-2 cells were treated with folic acid and poly (lactic acid) modified P85 / F127 and F87 nanoparticles (5 / 10 / L / L, 50 / 100, 200400 mg / L), compared with the control group. There was no significant difference (P0.05); (3). The results showed that the PLA-Pluronic-PLA nanoparticles and FA-Pluronic-PLA nanoparticles could be used as drug carriers when modified with folic acid and poly (lactic acid) triblock copolymers (P85 F127 and F87).
【作者單位】： 江西科技師范大學生命科學學院;
【基金】：國家自然科學基金項目(31360376,21264009) 江西省主要學科學術(shù)和技術(shù)帶頭人培養(yǎng)計劃(20153BCB 22009) 江西省高等學�？萍悸涞赜媱濏椖�(KJLD13071)~~
【分類號】：R99;TB383.1
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本文編號：2116857