本文選題：生物模板 + 卵清溶菌酶纖維　； 參考：《燕山大學(xué)》2015年碩士論文

【摘要】：卵清溶菌酶是一種單股肽鏈的堿性蛋白,在強(qiáng)酸中可以穩(wěn)定性的存在,但是當(dāng)加熱到一定溫度時(shí)便可以發(fā)生結(jié)構(gòu)變化,即形成網(wǎng)狀交錯(cuò)的納米纖維。卵清溶菌酶由于其自身的優(yōu)點(diǎn)引起了許多研究學(xué)者的興趣,現(xiàn)階段國(guó)內(nèi)外以卵清溶菌酶為模板組裝金屬納米粒子鏈的很少。本論文選用卵清溶菌酶纖維為生物模板,通過(guò)TEM、UV、EDS、SAED、XRD、CV表征手段對(duì)樣品進(jìn)行分析表征,本文主要研究了Pt納米粒子鏈、Rh納米粒子鏈、Au納米粒子鏈的組裝。主要完成內(nèi)容如下:1.通過(guò)TEM表征確定卵清溶菌酶及其纖維形貌結(jié)構(gòu)。2.通過(guò)討論不同條件下的對(duì)照實(shí)驗(yàn)探究出制備銠納米粒子鏈的最佳條件,并對(duì)其結(jié)果進(jìn)行分析,成功的制備出銠納米粒子鏈,纖維化4天組裝的銠納米粒子鏈具有兩種形態(tài),一種是長(zhǎng)在卵清溶菌酶纖維里面,其銠納米粒子鏈的直徑為30nm,一種是長(zhǎng)滿卵清溶菌酶纖維,其銠納米粒子鏈的直徑為40nm。纖維化4天時(shí)組裝的銠納米粒子鏈直徑為13nm,同時(shí)確定銠納米粒子鏈具有一定的催化活性。3.通過(guò)討論卵清溶菌酶的纖維化時(shí)間及p H條件確定最優(yōu)條件組裝鉑的納米粒子鏈,并通過(guò)EDS和SAED確定其屬于多晶型金屬;4.成功合成金納米粒子鏈�？疾炝瞬煌瑵舛鹊穆亚迦芫咐w維、加入化學(xué)體系后p H值及加入化學(xué)體系的溶液量對(duì)組裝金納米粒子鏈的影響。同時(shí)又通過(guò)改變其纖維化時(shí)間及最后階段的加熱條件來(lái)調(diào)控組裝的金納米粒子鏈的形貌,又通過(guò)MTT測(cè)試確定金納米粒子鏈具有較好的生物相容性,其在生物醫(yī)學(xué)界的應(yīng)用具有很大的潛在價(jià)值。
[Abstract]:Egg white lysozyme is a kind of basic protein with single strand peptide chain, which can be stable in strong acid. However, when heated to a certain temperature, the structure of egg white lysozyme can change, that is to say, it forms a network of interlaced nanofibers. Ovalbum lysozyme has attracted the interest of many researchers because of its own advantages. At present, few metal nanoparticles chains are assembled by using ovalbumin as template at home and abroad. In this paper, the ovalbum lysozyme fiber was selected as the biological template, and the samples were characterized by TEMUVUVSEDSSAEDX XRDX CV. In this paper, the assembly of au nanocrystalline chains of Pt nanocrystalline chain Rh nanoparticles was studied. The main contents are as follows: 1. The morphology and structure of lysozyme and its fiber were determined by TEM. The optimal conditions for the preparation of rhodium nanocrystalline chains were investigated by discussing the control experiments under different conditions and the results were analyzed. The rhodium nanoparticles chains were successfully prepared. The rhodium nanoparticles chains assembled in 4 days of fibrosis had two forms. The diameter of rhodium nanoparticle chain is 30 nm and the diameter of rhodium nanocrystalline chain is 40 nm. The diameter of the rhodium nanoparticles was 13 nm after 4 days of fibrosis. At the same time, it was determined that the rhodium nanoparticles chain had a certain catalytic activity. 3. By discussing the fibrosis time of ovalbum lysozyme and pH conditions, the optimal conditions for assembling platinum nanoparticles chain were determined, and the polycrystalline metal chain was determined by EDS and SAED. Gold nanoparticles chain was successfully synthesized. The effects of different concentrations of ovalbumin lysozyme fibers, pH value and the amount of solution added into the chemical system on the chain of gold nanoparticles were investigated. At the same time, the morphology of the assembled gold nanoparticles was regulated by changing the time of fibrosis and the heating conditions of the final stage, and the biocompatibility of the gold nanoparticles was determined by MTT test. Its application in biomedical field has great potential value.
【學(xué)位授予單位】：燕山大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：TB383.1

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本文編號(hào)：1986487