發(fā)布時(shí)間：2018-03-23 19:33

  本文選題：水熱制備法　切入點(diǎn)：生物量子點(diǎn)　出處：《南昌大學(xué)》2015年碩士論文

【摘要】：生物量子點(diǎn)(bio-dots)和熒光碳量子點(diǎn)(CQDs)是最近興起的新穎熒光納米材料,相對(duì)于傳統(tǒng)的半導(dǎo)體量子點(diǎn)而言,具有非常明顯的優(yōu)勢(shì)。除了光學(xué)性能優(yōu)良,bio-dots和CQDs還具有的毒性低、環(huán)境友好、制備方法簡(jiǎn)單、成本低等優(yōu)點(diǎn),讓它們?cè)诨瘜W(xué)和生物傳感領(lǐng)域有很大的應(yīng)用前景。本論文利用水熱方法成功制備了bio-dots和CQDs,通過(guò)對(duì)熒光量子點(diǎn)的表征,概述了其基本特征并深入探討了其在分析化學(xué)領(lǐng)域的應(yīng)用。研究的主要內(nèi)容如下:(1)以富含C堿基的DNA鏈作原料,通過(guò)低溫水熱法制備了熒光bio-dots。采用透射電鏡(TEM)、原子力顯微鏡(AFM)對(duì)bio-dots形貌進(jìn)行了表征,結(jié)果表明,其粒徑約為16 nm,厚度約為0.8 nm。用X-射線(xiàn)光電子能譜(XPS)對(duì)bio-dots成分進(jìn)行了分析,結(jié)果表明,bio-dots既保留了DNA的C堿基又與DNA的成分大致相同。利用熒光光譜(FL)對(duì)bio-dots進(jìn)行了光譜表征,其熒光發(fā)射波長(zhǎng)不隨激發(fā)波長(zhǎng)的變化而改變,當(dāng)激發(fā)波長(zhǎng)從260 nm增加320 nm時(shí),bio-dots的發(fā)射波長(zhǎng)均為420 nm。此外,實(shí)驗(yàn)結(jié)果表明bio-dots的耐光漂白性強(qiáng),然而pH對(duì)bio-dots的發(fā)光有較大影響。(2)基于bio-dots與Ag+的相互作用構(gòu)建光致發(fā)光傳感平臺(tái),用于谷胱甘肽(GSH)和谷胱甘肽還原酶(GR)活性的檢測(cè)。利用bio-dots與DNA結(jié)構(gòu)類(lèi)似的特性,以Ag+作為橋梁與bio-dots的C堿基配位形成C-Ag+-C配合物,從而破壞bio-dots的發(fā)光中心,導(dǎo)致bio-dots熒光猝滅。由于Ag+與巰基的配位能力比與C堿基更強(qiáng),當(dāng)加入GSH時(shí),Ag+首先與GSH結(jié)合,使bio-dots結(jié)構(gòu)不被破壞,因而熒光不猝滅,據(jù)此實(shí)現(xiàn)GSH定量分析。此外,基于GR-催化酶促反應(yīng)對(duì)GR活性和氧化型谷胱甘肽(GSSH)進(jìn)行了定量分析并用于抑制劑的篩選。(3)以尿酸作為碳源,180oC水熱反應(yīng)8 h即可獲得藍(lán)色熒光的CQDs。銅在許多生物進(jìn)程中被認(rèn)為是一種重要的微量元素,廣泛的參與生命的進(jìn)程,人體內(nèi)銅離子超標(biāo)會(huì)破壞人體中的代謝平衡產(chǎn)生一系列的疾病,如門(mén)克斯病、威爾遜病、阿爾茨海默病和普里昂疾病等。本文利用熒光CQDs表面豐富的氮、氧基團(tuán)與Cu2+發(fā)生配位作用,導(dǎo)致熒光猝滅,根據(jù)熒光猝滅程度對(duì)Cu2+進(jìn)行了定量分析,檢測(cè)Cu2+線(xiàn)性范圍為1-15μM。
[Abstract]:Biological quantum dots (bio-dotss) and fluorescent carbon quantum dots (CQDs) are novel fluorescent nanomaterials, which have obvious advantages over traditional semiconductor quantum dots. In addition to their excellent optical properties, bio-dots and CQDs have low toxicity. Due to their advantages of environmental friendliness, simple preparation method and low cost, they have great application prospects in chemical and biological sensing fields. In this paper, bio-dots and CQDswere successfully prepared by hydrothermal method, and characterized by fluorescence quantum dots. The main contents of this study are as follows: (1) the DNA chain rich in C bases is used as raw material. Fluorescence bio-dots were prepared by hydrothermal method at low temperature. The morphology of bio-dots was characterized by TEM and AFM. The results showed that the particle size was about 16 nm and the thickness was about 0.8 nm. The composition of bio-dots was analyzed by X-ray photoelectron spectroscopy (XPS). The results show that the C base of DNA is retained and the composition of bio-dots is about the same as that of DNA. The spectra of bio-dots are characterized by fluorescence spectra. The fluorescence emission wavelength does not change with the change of excitation wavelength. When the excitation wavelength is increased from 260 nm to 320 nm, the emission wavelengths of bio-dots are 420nm. In addition, the experimental results show that the photobleaching resistance of bio-dots is strong, but pH has a great effect on the luminescence of bio-dots. It is used for the detection of glutathione glutathione (GSH) and glutathione reductase (Glutathione reductase) activity. Using the similar structure of bio-dots and DNA, Ag is used as a bridge to coordinate with C base of bio-dots to form C-Ag C complex, which destroys the luminescent center of bio-dots. The fluorescence quenching of bio-dots is caused. Because Ag has stronger coordination ability with sulfhydryl than with C base, when GSH is added, Ag binds to GSH first, so the structure of bio-dots is not destroyed, thus the fluorescence is not quenched, so the quantitative analysis of GSH is realized. Gr activity and oxidized glutathione (GSSH) were quantitatively analyzed based on GR-catalyzed enzymatic reaction and were used to screen inhibitors. The blue fluorescence CQDs. copper was obtained by hydrothermal reaction of 180oC with uric acid as carbon source for 8 h. Cheng Zhong is considered to be an important trace element. With extensive participation in the process of life, excessive copper ions in the human body can disrupt the metabolic balance in the body and produce a range of diseases, such as Menkes disease, Wilson's disease, In this paper, the rich nitrogen and oxygen groups on the surface of fluorescent CQDs were used to coordinate with Cu2, which led to fluorescence quenching. The quantitative analysis of Cu2 was carried out according to the degree of fluorescence quenching. The linear range of detection of Cu2 was 1-15 渭 M.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：TB383.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前3條

1 王莉;呂婷;阮楓萍;鄧德剛;徐時(shí)清;;水熱法制備的熒光碳量子點(diǎn)[J];發(fā)光學(xué)報(bào);2014年06期

2 袁鳴;鐘睿博;張景偉;李潔;張盼;馬宇星;張萍;韓小寧;張峰;;熒光碳點(diǎn)在生物醫(yī)學(xué)領(lǐng)域中的應(yīng)用研究進(jìn)展[J];基因組學(xué)與應(yīng)用生物學(xué);2015年01期

3 唐志姣;李攻科;胡玉玲;;熒光碳點(diǎn)在分析檢測(cè)中的研究進(jìn)展[J];分析測(cè)試學(xué)報(bào);2015年08期

相關(guān)碩士學(xué)位論文 前3條

1 雒連春;熒光碳納米粒子的微波加熱法合成、表征及在細(xì)胞成像中的應(yīng)用[D];陜西師范大學(xué);2013年

2 趙永強(qiáng);基于單一基碳點(diǎn)白光熒光粉的LED[D];太原理工大學(xué);2015年

3 何玉珩;白光LED用單一基質(zhì)碳點(diǎn)基熒光粉的制備與表征[D];太原理工大學(xué);2015年

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本文編號(hào)：1654870