本文關(guān)鍵詞： 碳點(diǎn) 纖維素 生物成像 Fe(Ⅲ)檢測(cè)　出處：《浙江理工大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：碳點(diǎn)(CDs)是一種尺寸小于10 nm的新型熒光納米粒子,自2004年CDs被報(bào)道以來,已經(jīng)吸引了廣大科研工作者的興趣和關(guān)注。CDs具有極好的生物相容性、優(yōu)秀的熒光性能、穩(wěn)定的化學(xué)和物理性質(zhì)、低毒性、極佳的水溶性等性質(zhì),大大拓展了CDs的應(yīng)用領(lǐng)域和范圍。目前,CDs的制備方法主要有,激光蝕刻法,電化學(xué)法,模板法等,這些方法存在原料價(jià)格高,設(shè)備價(jià)格昂貴或者實(shí)驗(yàn)操作復(fù)雜等缺陷,因此,急需一種操作簡(jiǎn)單,原料便宜同時(shí)可提高碳點(diǎn)熒光量子產(chǎn)率的制備方法。提高CDs熒光量子產(chǎn)率的方法一般有兩種,一種是后期對(duì)CDs進(jìn)行表面鈍化;另一種是摻雜雜原子。為了進(jìn)一步降低制備成本,改善CDs的熒光性能,本課題選擇以廉價(jià)易得可降解的生物質(zhì)基—纖維素為原料,摻雜氮硫雜原子,通過一步水熱法制得一系列纖維素基熒光CDs,分別對(duì)制得的CDs進(jìn)行最適實(shí)驗(yàn)條件探究,形貌表征,成分和性能分析,通過細(xì)胞毒性試驗(yàn)評(píng)價(jià)其毒性,并將它們應(yīng)用于生物成像與在溶液中對(duì)Fe(Ⅲ)檢測(cè),并對(duì)Fe(Ⅲ)檢測(cè)下限和檢測(cè)機(jī)理進(jìn)行探究最終得到實(shí)驗(yàn)結(jié)論如下:(1)氮摻雜纖維素基熒光CDs的制備及在生物成像的應(yīng)用研究:以纖維素粉和尿素為原料,通過綠色環(huán)保的水熱法一步制備了氮摻雜纖維素基熒光CDs(N-CDs)�？疾炝四蛩刭|(zhì)量、反應(yīng)時(shí)間和反應(yīng)溫度對(duì)N-CDs熒光量子產(chǎn)率的影響,并探究最適實(shí)驗(yàn)條件為:尿素與纖維素的質(zhì)量比為0.7,反應(yīng)溫度為180°C,反應(yīng)時(shí)間為72 h,此時(shí)N-CDs的熒光量子產(chǎn)率達(dá)21.7%;對(duì)N-CDs的形貌、成分和光學(xué)性能進(jìn)行表征與分析,發(fā)現(xiàn)它的平均尺寸為4.2 nm,表面存在氨基、羥基等基團(tuán),具有依賴激發(fā)光波長(zhǎng)的熒光性質(zhì);通過細(xì)胞毒性試驗(yàn),發(fā)現(xiàn)N-CDs的細(xì)胞存活率達(dá)89%以上,并將其成功應(yīng)用于細(xì)胞成像。(2)氮硫共摻雜纖維素基熒光CDs的制備及在生物成像的應(yīng)用研究:以纖維素粉、硫代硫酸鈉和乙二胺(EDA)為原料,通過綠色環(huán)保的水熱法一步制備了氮硫共摻雜纖維素基熒光CDs(N,S-CDs)。N,S-CDs的最適實(shí)驗(yàn)條件為:纖維素粉0.5 g,硫代硫酸鈉0.1 g,乙二胺0.5 mL,反應(yīng)時(shí)間72 h,反應(yīng)溫度180°C,此時(shí)他的熒光量子產(chǎn)率達(dá)28.2%;并對(duì)N,S-CDs的形貌、成分和光學(xué)性能進(jìn)行表征與分析,發(fā)現(xiàn)它的平均尺寸為3.2 nm,存在氨基,羥基和含硫基團(tuán)等基團(tuán),具有依賴激發(fā)光波長(zhǎng)的熒光性質(zhì);相較于N-CDs,N,S-CDs具有更高的熒光量子產(chǎn)率,相同激發(fā)光下的N,S-CDs發(fā)射光發(fā)生紅移;通過細(xì)胞毒性試驗(yàn),發(fā)現(xiàn)N,S-CDs的細(xì)胞存活率達(dá)93%以上,并將其成功應(yīng)用于細(xì)胞成像。(3)纖維素基熒光CDs在溶液中對(duì)Fe(Ⅲ)檢測(cè)的應(yīng)用研究:將Cellulose-CDs,N-CDs,N,S-CDs和EDA-CDs分別在PBS緩沖液和水溶液當(dāng)中進(jìn)行Fe(Ⅲ)檢測(cè)。并對(duì)N,S-CDs和EDA-CDs分別在PBS緩沖液和水溶液中進(jìn)行Fe(Ⅲ)檢測(cè)下限的測(cè)定,分別為0.3119 ppm和0.03378 ppm,對(duì)其檢測(cè)機(jī)理進(jìn)行探究。
[Abstract]:Carbon dots (CDs) is a new type of fluorescent nanoparticles with size less than 10 nm, which has been reported since 2004. CDs have attracted the interest and attention of many researchers. CDs have excellent biocompatibility, excellent fluorescence properties, stable chemical and physical properties, low toxicity, excellent water solubility and so on. At present, the main preparation methods of CDs are laser etching, electrochemical method, template method and so on. These methods have high raw material price. The equipment is expensive or the experiment operation is complex, so it is urgent to have a simple operation. The method of preparing carbon dot fluorescence quantum yield can be improved by cheap raw material. There are two methods to improve CDs fluorescence quantum yield, one is to passivate the surface of CDs in the later stage; In order to further reduce the cost of preparation and improve the fluorescence properties of CDs, we choose biodegradable biomass-based cellulose as the raw material, doped nitrogen and sulfur hetero atoms. A series of Cellulose based fluorescent CDs were obtained by one step hydrothermal method. The optimal experimental conditions, morphology characterization, composition and performance analysis of the CDs were studied, and the toxicity was evaluated by cytotoxicity test. They were applied to biometric imaging and detection of Fe (鈪，

本文編號(hào)：1450466