本文關鍵詞：海藻酸鈣復合多孔薄膜的制備及其電化學研究　出處：《青島大學》2015年碩士論文　論文類型：學位論文

  更多相關文章： 海藻酸鈣復合多孔薄膜 聚乙烯醇 甘油 明膠 電化學

【摘要】：對第三代新型電化學生物傳感器的構造及優(yōu)化是本論文研究的主要內容,選用合適的新材料用于構造更加新穎且實用的電化學生物傳感界面,使蛋白質、酶在修飾電極表面的電化學方面的性質得以更好地研究。為此,本文在原有多孔海藻酸鈣(CA)膜修飾電極的基礎上通過引入新物質,探究了肌紅蛋白(Mb)如何更加穩(wěn)定的通過薄膜固定在電極表面,構造出一種含水率高、穩(wěn)定性好、電化學性能優(yōu)良的電化學生物傳感器,并且通過一系列的表征和電化學測試對其進行研究。具體內容如下:具有良好成膜性的海藻酸鈉(SA)結合制孔劑聚乙二醇(PEG)已成功制備出多孔CA膜,在此基礎上,為了得到一種穩(wěn)定性更好、含水率更高的薄膜,將聚乙烯醇(PVA)、甘油和明膠引入多孔CA膜,從而構造出CA’s復合多孔薄膜。將CA’s復合多孔薄膜涂布于玻碳電極上,然后將Mb固載于此復合多孔薄膜修飾電極便成功制備出一種具有良好電化學性能的Mb-CA’s復合多孔薄膜修飾電極。利用掃描電子顯微鏡(SEM)、紫外-可見吸收光譜(UV-vis)、交流阻抗(EIS)、循環(huán)伏安(CV)、方波伏安(SWV)、計時安培等方法分別對所構造的復合多孔薄膜進行表征和性能測定。UV-vis表明Mb在所構造的CA’s復合多孔薄膜中依然保持了其原有的二級結構。EIS數(shù)據(jù)反映出加入PVA、甘油、明膠三種新物質后,電荷傳遞電阻(Rct)明顯下降。CV曲線中在㧟0.37 V(vs SCE)處出現(xiàn)了蛋白質血紅素FeIII/II電對的可逆氧化還原峰。對SWV法得到的數(shù)據(jù)進行擬合獲得了表觀異相電子傳遞速率常數(shù)(ks)及氧化還原式電勢(Eο?)。Mb-CA’s復合多孔薄膜修飾電極在催化氧氣(O2)、過氧化氫(H2O2)和亞硝酸鈉(NaNO2)時均表現(xiàn)出良好的電催化性能,穩(wěn)定性好,靈敏度高,檢測限低。
[Abstract]:The structure and optimization of the third generation new electrochemical biosensor is the main content of this paper. The suitable new materials are selected to construct a more novel and practical electrochemical biosensor interface to make protein. The electrochemical properties of enzyme on the surface of modified electrode can be better studied. Therefore, a new material was introduced into the modified electrode based on the original porous calcium alginate CAA membrane modified electrode. This paper explores how myoglobin Mb) is more stable to be fixed on the electrode surface through thin film to construct an electrochemical biosensor with high moisture content, good stability and excellent electrochemical performance. It was studied by a series of characterization and electrochemical tests. The main contents are as follows: sodium alginate with good film forming property combined with polyethylene glycol (PEG). Porous CA membranes have been successfully prepared. On this basis, in order to obtain a film with better stability and higher moisture content, polyvinyl alcohol PVA, glycerol and gelatin were introduced into porous CA membrane. The CA's composite porous film was constructed and the CA's composite porous film was coated on the glassy carbon electrode. Then a Mb-CA's composite porous film modified electrode with good electrochemical performance was successfully prepared by immobilization of Mb on the composite porous film modified electrode. UV-Vis absorption spectra, AC impedance, cyclic voltammetry (CV), square wave voltammetry (SWV). The composite porous films were characterized and characterized by chronoamperometric method. UV-vis showed that Mb still maintained its original secondary structure in the CA's composite porous films. EIS data reflect the addition of PVA. After glycerin, gelatin three new substances, charge transfer resistance (Rct) significantly decreased in the. CV curve in? 0.37 VX vs SCE). The reversible redox peak of protein heme FeIII/II pair appeared. The apparent heterogeneous electron transfer rate constant was obtained by fitting the data obtained by SWV method. And redox potential? The modified electrode has good electrocatalytic performance and good stability when it is used to catalyze O _ 2O _ 2 H _ 2O _ 2 (H _ 2O _ 2 H _ 2O _ 2) and Na _ 2O _ 2 (Na _ (2) O _ (2) O _ (2) O _ (2) H _ (2) O _ (2)). High sensitivity and low detection limit.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.2

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本文編號：1427776