伴隨著我國(guó)的城市化進(jìn)程加快,建筑行業(yè)迅速發(fā)展。混凝土成為了世界上用途最廣、用量最大的建筑材料,而其主要成分的水泥并不是一種可持續(xù)發(fā)展的膠結(jié)材料。每生產(chǎn)1噸水泥就會(huì)排放近1噸CO₂,嚴(yán)重影響了城市的生態(tài)環(huán)境。我國(guó)已把推進(jìn)建筑業(yè)可持續(xù)發(fā)展作為節(jié)能減排的重要內(nèi)容,尋找生態(tài)環(huán)境材料是發(fā)展低碳建筑的內(nèi)在要求。而自然的生態(tài)智慧可用于解決建筑材料與維護(hù)生態(tài)環(huán)境之間的矛盾。許多天然生物材料,比如珊瑚、骨骼、牙齒和貝殼等,是生物礦化過(guò)程中形成的鈣質(zhì)陶瓷和高分子復(fù)合物。這為建筑材料的可持續(xù)發(fā)展提供了一種新思路。某些微生物可用于模擬自然界的礦化過(guò)程,產(chǎn)生具有膠結(jié)作用的碳酸鈣沉積。這類(lèi)微生物主要是產(chǎn)脲酶菌,其礦化產(chǎn)物被稱(chēng)為“生物水泥”,這一生物礦化過(guò)程也被稱(chēng)為Microbially Induced calcium Carbonate Precipitation（MICP）。本文圍繞基于MICP的生物礦化過(guò)程在可持續(xù)建筑材料中的應(yīng)用潛力這一中心問(wèn)題,研究了生物水泥修復(fù)受損建筑材料以延長(zhǎng)服役壽命,探索了生物水泥與輔助性膠凝材料（偏高嶺土、粉煤灰）復(fù)合用于降低水泥基材料中水泥的含量以及改良土體... 

【文章來(lái)源】：華東師范大學(xué)上海市 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：156 頁(yè)

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
Abstract
Abbreviations
Chapter 1: Introduction
    1.1 General Introduction
    1.2 Ecological wisdom in nature for sustainable building materials
    1.3 Microbially induced calcium carbonate precipitation
    1.4 Problem and gap in studies
    1.5 Research objectives
    1.6 Thesis organization
Chapter 2: Literature Review
    2.1 Microbial activities leading to carbonate precipitation
    2.2 MICP process driven by urease enzyme
    2.3 Factors affecting the efficiency of MICP
    2.4 Urease producing bacterial isolation source
    2.5 Polymorphism of carbonate crystals
    2.6 Production of MICP: Biocement
    2.7 Biocement and properties of building materials
    2.8 Applications of MICP in building materials
        2.8.1 Biocement in remediation of building materials
        2.8.2 Biocement in low energy building materials
        2.8.3 MICP in ground improvement
    2.9 Summary of literature review and future prospective
Chapter 3: Complete bacterial community analysis of Yixing Shanjuan Cave and bio-consolidation of cracks in masonry cement mortars by one of urease producing isolate
    3.1 Introduction
    3.2 Materials and methods
        3.2.1 Sample collection
        3.2.2 Bacterial community analysis using Illumina Mi Seq
            3.2.2.1 Total DNA extraction and DNA sequence analysis
            3.2.2.2 Bioinformatics analysis
        3.2.3 Isolation and characterization analysis
            3.2.3.1 Bacterial isolation
            3.2.3.2 Identification of best urease producing bacteria
            3.2.3.3 Optimization of conditions for urease activity
            3.2.3.4 Bacterial growth profile and p H profile
            3.2.3.5 Urease activity
            3.2.3.6 Calcite estimation
        3.2.4 Bio-consolidation of cracks in masonry cement mortars
            3.2.4.1 Biocement production
            3.2.4.2 Mortar preparation and crack generation
            3.2.4.3 Consolidation of cracks
            3.2.4.4 Water absorption
            3.2.4.5 Compressive strength
            3.2.4.6 Micro-structural analyses
            3.2.4.7 Thermogravimetric and differential scanning calorimetry
    3.3 Results and Discussion
        3.3.1 Microbial diversity of Yixing Shanjuan karst cave of China
        3.3.2 Isolation and characterization analysis
            3.3.2.1 Isolation and identification of best ureolytic isolate
            3.3.2.2 Optimization of conditions for urease activity
            3.3.2.3 Bacterial growth and p H profiles
            3.3.2.4 Urease activity and calcite estimation
        3.3.3 Bio-consolidation of cracks in masonry cement mortars
            3.3.3.1 Compressive strength
            3.3.3.2 Water absorption
            3.3.3.3 Micro-structural analyses
            3.3.3.4 Thermogravimetric analysis
    3.4 Conclusion
Chapter 4: Improvement in the performance and properties of cement mortars with secondary cementitious material by biomineralization
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Sample collection
        4.2.2 Isolation and identification of urease producing bacterium
        4.2.3 Materials
        4.2.4 Biocement development
        4.2.5 Biomineralization in MK
        4.2.6 Mortar specimens preparation with MK
        4.2.7 Porosity of mortar specimens
        4.2.8 Micro-structural analyses
    4.3 Results and discussion
        4.3.1 Urease producing bacterium
        4.3.2 Compressive strength
        4.3.3 Porosity
        4.3.4 SEM-EDS
        4.3.5 FTIR
        4.3.6 XRD
    4.4 Conclusions
Chapter 5: Fly ash incorporated with biocement to improve engineering properties of expansive soil
    5.1 Introduction
    5.2 Materials and Methods
        5.2.1 Materials
        5.2.2 Biocement production
        5.2.3 Sample preparation
        5.2.4 Atterberg limits
        5.2.5 Free swell testing method
        5.2.6 Unconfined Compressive Strength （UCS） test
        5.2.7 Micro-structural analyses
    5.3 Results and Discussion
        5.3.1 Atterberg limits
        5.3.2 Swelling potential
        5.3.3 Unconfined Compressive Strength （UCS）
        5.3.4 SEM-EDX
        5.3.5 FTIR and XRD
    5.4 Conclusions
Chapter 6: Bio-grout based on microbially induced sand solidification by means of asparaginase activity
    6.1 Introduction
    6.2 Materials and Methods
        6.2.1 Materials
        6.2.2 Asparaginase assay
        6.2.3 Bio-grout preparation
        6.2.4 Strength and permeability of Bio-grout
        6.2.5 Micro-structural analyses
        6.2.6 X-ray computed tomography （XCT）
        6.2.7 Thermogravimetry analysis （TGA）
    6.3 Results
        6.3.1 Asparaginase activity
        6.3.2 Mechanical properties
        6.3.3 SEM-EDS analysis
        6.3.4 XRD and XCT
        6.3.5 Thermogravimetric analysis （TGA）
    6.4 Discussion
Chapter 7: Conclusion, Innovation and Future Perspectives
    7.1 Conclusion
    7.2 Innovation
    7.3 Future perspectives
References
Appendix: Complete genome sequence of carbonic anhydrase producing Psychrobacter sp. SHUES
致謝
攻讀博士學(xué)位期間發(fā)表的論文



本文編號(hào)：3010749