發(fā)布時間：2018-11-02 17:48

【摘要】：目的:耐力訓(xùn)練引起的高脂膳食誘導(dǎo)肥胖大鼠Irisin水平變化與脂肪組織FTO表達的關(guān)系研究。方法:以雄性SD大鼠為研究對象,將大鼠隨機分為對照組(C組)、高脂膳食組(HD)、高脂膳食結(jié)合運動組(HDE)和運動訓(xùn)練組(CE組),以跑臺訓(xùn)練為運動干預(yù)手段,坡度為0°,8.2m/min(相當于53%VO2max),第1次運動15min,第2次運動30min,第3次運動45min,第4次以后每次運動60min,共運動8周,每周5次,測定大鼠體重、脂肪重量、血液Irisin含量、骨骼肌FNDC5mRNA和蛋白表達、脂肪組織FTO和UCP1基因mRNA和蛋白表達。結(jié)果:高脂膳食顯著引起大鼠體重和脂肪含量增加,血液Irisin水平顯著降低,骨骼肌FNDC5表達顯著降低,脂肪組織FTO表達顯著升高而UCP1表達顯著降低;耐力訓(xùn)練可以顯著降低高脂膳食引起的體重和脂肪含量的增加,顯著升高高脂膳食引起的血液Irisin降低,顯著升高高脂膳食誘導(dǎo)的骨骼肌FNDC5表達的降低,顯著降低高脂膳食引起的脂肪組織FTO表達的升高,而顯著升高高脂膳食誘導(dǎo)的UCP1表達的降低;血液中Irisin水平與脂肪組織中FTOmRNA和蛋白呈中度負相關(guān);高脂膳食與高脂膳食結(jié)合運動組,骨骼肌中FNDC5mRNA和蛋白與脂肪組織FTOmRNA和蛋白分別呈中度負相關(guān);運動組FNDCmRNA與脂肪組織FTOmRNA無相關(guān),而蛋白呈中度負相關(guān)。結(jié)論:耐力訓(xùn)練大鼠可能通過釋放Irisin,抑制脂肪組織FTO基因表達,進而增強UCP1表達。
[Abstract]:Aim: to study the relationship between the changes of Irisin level and the expression of FTO in adipose tissue of obese rats induced by high fat diet induced by endurance training. Methods: male SD rats were randomly divided into control group (group C), high fat diet group (HD), group) combined with high fat diet group (HDE) group) and exercise training group (CE group). The slope was 0 擄, 8.2m/min (equivalent to 53%VO2max), the first exercise was 15 minutes, the second exercise was 30 minutes, the third exercise was 45 minutes, the fourth time was 60 minutes, the total exercise was 8 weeks, 5 times a week. The weight and fat weight of the rats were measured. Blood Irisin content, skeletal muscle FNDC5mRNA and protein expression, adipose tissue FTO and UCP1 gene mRNA and protein expression. Results: high fat diet significantly increased body weight and fat content, decreased blood Irisin level, significantly decreased FNDC5 expression in skeletal muscle, increased FTO expression in adipose tissue and decreased UCP1 expression in adipose tissue. Endurance training significantly decreased the increase of body weight and fat content induced by high fat diet, significantly increased the decrease of blood Irisin induced by high fat diet, and significantly increased the expression of FNDC5 in skeletal muscle induced by high fat diet. The increase of FTO expression in adipose tissue induced by high fat diet was significantly decreased, while the decrease of UCP1 expression induced by high fat diet was significantly increased. The level of Irisin in blood was negatively correlated with FTOmRNA and protein in adipose tissue, FNDC5mRNA and protein in skeletal muscle were negatively correlated with FTOmRNA and protein in adipose tissue in high fat diet and high fat diet combined exercise group. In exercise group, there was no correlation between FNDCmRNA and FTOmRNA in adipose tissue, but there was a moderate negative correlation between FTOmRNA and protein in exercise group. Conclusion: endurance training rats may inhibit the expression of FTO gene in adipose tissue by releasing Irisin, and then enhance the expression of UCP1.
【作者單位】： 山東理工大學(xué)體育學(xué)院;
【基金】：山東理工大學(xué)青年支持計劃項目“耐力訓(xùn)練改善肥胖的FTO機制研究”(110026)
【分類號】：G804.2

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