【摘要】：目的:大電導(dǎo)鈣激活鉀通道(BK_(Ca))對神經(jīng)元興奮性以及動作電位發(fā)放頻率具有重要調(diào)控作用,擬觀察肌酸補充對皮層神經(jīng)元葡萄糖剝奪時BK_(Ca)電流密度及胞內(nèi)鈣離子濃度的影響。方法:全細胞膜片鉗技術(shù)記錄肌酸干預(yù)對不同時長葡萄糖剝奪原代培養(yǎng)大鼠皮層神經(jīng)元BK_(Ca)電流的變化;應(yīng)用激光共聚焦檢測神經(jīng)元胞內(nèi)游離鈣離子濃度。結(jié)果:1)葡萄糖剝奪1~2 h BK_(Ca)電流密度顯著增加(P0.01),4~5 h顯著減小(P0.01);胞內(nèi)游離Ca~(2+)熒光強度隨葡萄糖剝奪1~5 h逐漸增強(P0.01)。2)肌酸孵育顯著減小原代培養(yǎng)神經(jīng)元BK_(Ca)電流密度以及胞內(nèi)游離Ca~(2+)熒光強度(P0.01)。結(jié)論:葡萄糖剝奪1~2 h使胞內(nèi)游離Ca2+濃度顯著升高,激活BK_(Ca)大量開放,使神經(jīng)元超極化,降低神經(jīng)元興奮性;Cr可有效抑制葡萄糖剝奪引起B(yǎng)K_(Ca)電流增加以及胞內(nèi)鈣離子濃度升高,維持錐體神經(jīng)元的興奮性。
[Abstract]:Aim: to investigate the effects of creatine supplementation on BK_ (Ca) current density and intracellular calcium concentration in cortical neurons after glucose deprivation. Methods: whole-cell patch clamp technique was used to record the changes of BK_ (Ca) currents in primary cultured rat cortical neurons exposed to glucose deprivation for different periods, and the intracellular free calcium concentration of neurons was detected by confocal laser. Results: 1) the current density of BK_ (Ca) increased significantly (P0.01) at 1h of glucose deprivation and decreased significantly at 4h (P0.01), and the fluorescence intensity of intracellular free Ca~ (2) increased gradually with 1h of glucose deprivation (P0.01). 2) the current density of BK_ (Ca) in primary cultured neurons was significantly decreased by creatine incubation. And intracellular free Ca~ (2) fluorescence intensity (P0.01). Conclusion: 1h glucose deprivation can significantly increase the concentration of intracellular free Ca2, activate BK_ (Ca), hyperpolarization and decrease the excitability of neurons, Cr can effectively inhibit the increase of BK_ (Ca) current and the increase of intracellular Ca ~ (2 +) concentration induced by glucose deprivation. Maintain excitability of pyramidal neurons.
【作者單位】： 北京體育大學(xué)運動人體科學(xué)學(xué)院;北京體育大學(xué)教育部運動與體質(zhì)健康重點實驗室;
【基金】：國家自然科學(xué)基金資助項目(編號:31271278) 中央高�；究蒲袠I(yè)務(wù)費專項資金資助項目(編號:2016RC004)
【分類號】：G804.7
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本文編號：2281488