【摘要】：目的:急性大強度運動是一種劇烈運動,會對運動負荷產(chǎn)生氧化應(yīng)激。本研究以健康成年男性為研究對象,基于核磁共振(NMR)代謝組學(xué)方法對受試者進行急性大強度運動后不同時間段的血漿進行代謝組學(xué)分析,探討其代謝特征,旨在明確運動對體內(nèi)代謝物的影響,發(fā)現(xiàn)與運動密切相關(guān)的代謝化合物,從代謝物層面為研究運動監(jiān)控的相關(guān)問題提供新思路,為科學(xué)安排運動訓(xùn)練提供參考。方法:選取山西大學(xué)2014級體育教育專業(yè)男生10名(經(jīng)篩選后保留8人),全力騎行功率自行車30s×3/間歇3min,負荷為0.075Kg/Kg體重。分別于清晨8點安靜時、運動后即刻、運動后1小時、運動后1天、運動后1周進行肘靜脈采血5mL(除運動后即刻與1小時之外,其余全為清晨空腹采血),標記為E1、E2、E3、E4、E5。采集的血液經(jīng)離心、去蛋白等處理后進行1H-NMR檢測。采用Mest ReNova、SIMCA-P11.0、SPSS17.0軟件等進行處理與分析并找出差異代謝標志物。結(jié)果:(1)基于NMR代謝組學(xué)技術(shù)研究,人體在急性大強度運動后即刻與安靜狀態(tài)時代謝物相比代謝物存在顯著性差異,本研究共發(fā)現(xiàn)13種代謝差異物(VIP1且P0.01):包括亮氨酸、異亮氨酸、纈氨酸、乳酸、谷氨酸、谷氨酰胺、肌酸、肉毒堿、甜菜堿、乙酰乙酸、甘氨酸、甘油、葡萄糖,這幾種代謝產(chǎn)物在運動后即刻積分值只有乳酸呈上升趨勢,其余均呈下降趨勢。(2)運動后1小時與運動前相比的差異代謝標志物共有8種,分別是:亮氨酸、異亮氨酸、纈氨酸、乳酸、甜菜堿、乙酰乙酸、甘油(P0.01)、肌酸(P0.05),這幾種代謝產(chǎn)物除乳酸趨勢向上外,其余代謝物均依舊趨勢向下。運動后1天與運動前相比的差異代謝標志物只有甜菜堿(P0.05)呈下降趨勢,運動后1周與運動前相比已無顯著性差異。結(jié)論:(1)在急性大強度運動干預(yù)下,運動后即刻與運動前的差異代謝標志物共有13種,只有乳酸呈上升趨勢,約為運動前的3倍,但可恢復(fù)。(2)在急性大強度運動干預(yù)下,運動后人體無氧代謝供能系統(tǒng)的磷酸原系統(tǒng)、糖酵解系統(tǒng)以及脂肪代謝、氨基酸代謝等過程產(chǎn)生一系列變化,并且這些指標在1周內(nèi)基本恢復(fù)。
[Abstract]:Objective: acute high-intensity exercise is a violent exercise that produces oxidative stress on exercise load. In this study, the plasma metabolites of healthy adult men at different time periods after acute high-intensity exercise were analyzed based on (NMR) metabonomics, and the metabolic characteristics of plasma were investigated. The aim of this paper is to clarify the effect of exercise on metabolites in vivo, to find the metabolites closely related to exercise, to provide a new idea for the study of the related problems of exercise monitoring from the metabolite level, and to provide a reference for the scientific arrangement of exercise training. Methods: a total of 10 male students (8 of whom were retained after selection) were selected from 2014 P. E. major in Shanxi University. The bicycle was cycled for 30 seconds 脳 3 / 3 / interval for 3 mins, and the load was 0.075Kg/Kg weight. 5mL was collected from the elbow vein 1 week after exercise at 8 am at rest, immediately after exercise, 1 hour after exercise and 1 week after exercise (except immediately after exercise and 1 hour after exercise, all of which were collected on an empty stomach in the morning). The collected blood was tested for 1H-NMR after centrifugation, deproteinization and other treatments. SIMCA-P11.0 SPSS 17.0 software was used to process and analyze the differential metabolic markers. Results: (1) based on the study of NMR metabolomics, there were significant differences in metabolites between human body and those in quiet state immediately after acute intensive exercise. There were 13 metabolites (VIP1 and P0.01): leucine and isoleucine were found in this study. Valine, lactic acid, glutamine, creatine, carnitine, betaine, acetoacetic acid, glycine, glycerol, glucose, these metabolites showed an upward trend at the instant after exercise. (2) there were 8 different metabolic markers (leucine, isoleucine, valine, lactic acid, betaine, acetoacetic acid) between 1 hour after exercise and 1 hour after exercise. Glycerol (P0.01), creatine (P0.05), these metabolites except lactate trend upward, the other metabolites are still downward trend. Only betaine (P0.05) showed a decreasing trend 1 day after exercise compared with before exercise, and there was no significant difference between before exercise and 1 week after exercise. Conclusion: (1) under the intervention of acute high intensity exercise, there were 13 different metabolic markers immediately after exercise and before exercise, only lactic acid showed an upward trend, about 3 times as much as before exercise, but could recover. (2) under the intervention of acute high intensity exercise, there were 13 different metabolic markers. After exercise, a series of changes were found in the prophosphate system, glycolysis system, fat metabolism, amino acid metabolism and so on in the anaerobic energy supply system of human body, and these indexes basically recovered within one week.
【學(xué)位授予單位】：山西大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.7

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