本文選題：向心運動 + 離心運動�。� 參考：《河北師范大學(xué)》2017年碩士論文

【摘要】：肥胖全球流行已成當前公共健康問題,防肥減肥任務(wù)重大。其中運動對減肥的防控也是研究熱點之一。PGC-1α是細胞能量代謝與脂肪氧化調(diào)節(jié)的關(guān)鍵信號,探索不同的運動方式對PGC-1α的激活,對運動減肥方案的制定具有重要的意義。研究目的:觀察在相同攝氧量強度下,不同肌肉收縮形式(向心和離心)的運動對PGC-1α的激活作用,進而揭示相同攝氧量狀態(tài)下,不同運動方式對運動后0-48小時內(nèi)機體能量代謝信號的作用,以及是否存在時相性變化。研究方法:實驗第一部分取雄性SD大鼠24只,隨機分為向心運動組(E組)和離心運動組(L組),各組分別為12只大鼠。先進行三天的適應(yīng)性訓(xùn)練后,休息24小時。E組大鼠進行平坡跑運動(坡度0°,跑速20m/min,時間15min),L組大鼠進行下坡跑運動(坡度-5°,跑速Xm/min,時間15min)。實驗中調(diào)整L組的跑速直至攝氧量與E組一致。所得到的跑速為實驗第二部分L組大鼠的跑速X。實驗第二部分取雄性SD大鼠66只,隨機分成11組,每組6只,分別為對照組(C),向心運動后E0、E6、E12、E24、E48小時組。離心運動后L0、L6、L12、L24、L48小時組。向心運動組和離心運動組分別進行三天的適應(yīng)性訓(xùn)練。休息24小時。E組大鼠進行平坡跑運動(坡度0°、跑速20m/min,時間60min)。L組大鼠進行下坡跑運動(坡度-5°、跑速Xm/min,時間60min)。跑速X為實驗第一部分確定的跑速。各組大鼠一次性跑臺運動后取材,采用Western Blot檢測腓腸肌和比目魚肌中PGC-1α的蛋白達情況,采用透射電子顯微鏡觀察大鼠比目魚肌肌纖維微細結(jié)構(gòu)的變化。研究結(jié)果:(1)E組與L組大鼠在跑速為20m/min時攝氧量無統(tǒng)計學(xué)差異(P0.05)。(2)L組肌纖維微細結(jié)構(gòu)的變化有大于E組的趨勢,且持續(xù)的時間較長。(3)E組與L組大鼠在相同攝氧量強度下不同肌肉收縮方式(向心和離心)運動后,腓腸肌和比目魚肌PGC-1α蛋白表達量與C組相比,E組均有增加,但無統(tǒng)計學(xué)差異(P0.05),L組PGC-1α蛋白表達量明顯增加,且在0h、48h具有非常顯著性差異(P0.01),但在6h、24h無統(tǒng)計學(xué)差異(P0.05),在12h具有顯著性差異(P0.05)。E組腓腸肌和比目魚肌PGC-1α蛋白表達量與L組相比,在0h、12h、48h具有顯著性差異(P0.05),在6h、24h無統(tǒng)計學(xué)差異(P0.05)。結(jié)論:攝氧量相同的一次性跑臺運動后,離心收縮可顯著性提升大鼠骨骼肌PGC-1α蛋白的表達,且持續(xù)時間較長,呈現(xiàn)運動后即刻和48小時雙高峰現(xiàn)象。而向心收縮有增加趨勢但無統(tǒng)計學(xué)意義。
[Abstract]:Obesity has become a global public health problem and the task of preventing obesity and losing weight is very important. Among them, the prevention and control of exercise on weight loss is also one of the research hotspots. PGC-1 偽 is the key signal of cell energy metabolism and lipid oxidation regulation. It is of great significance to explore the activation of PGC-1 偽 in different exercise modes and to make the exercise weight loss program. Objective: to observe the activation of PGC-1 偽 by the exercise of different muscle contraction forms (concentric and centrifugal) under the same oxygen uptake intensity. The effects of different exercise modes on energy metabolism signal in 0-48 hours after exercise, and whether there are temporal changes. Methods: in the first part of the experiment, 24 male Sprague-Dawley rats were randomly divided into two groups: concentric exercise group (n = 12) and centrifugal exercise group (n = 12). After three days of adaptive training, the rats in group E were given downhill running (slope 0 擄, running speed 20 m / min, time 15 min) and downhill running (slope -5 擄, running speed X m / min, time 15 min). In the experiment, the running speed of group L was adjusted until oxygen uptake was the same as that in group E. The second part of the experiment was the running speed of the L group rats. In the second part, 66 male Sprague-Dawley rats were randomly divided into 11 groups with 6 rats in each group. After centrifugation, L _ 0, L _ 6, L _ (12) and L _ (24) L _ (48) h group. The centripetal exercise group and the centrifugal exercise group were trained for 3 days respectively. The rats in the rest group (0 擄slope, 20 m / min running speed) underwent downhill running (slope -5 擄, running speed X m / min, time 60 mins) in the rest group (0 擄, 20 m / min), while the rats in the 60min).L group underwent downhill running (slope -5 擄, running speed X m / min, time 60 min). Running speed X is the speed determined in the first part of the experiment. The contents of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle were detected by Western Blot and the changes of fiber microstructures in soleus muscle were observed by transmission electron microscope. Results there was no significant difference in oxygen uptake between group E and group L when the running speed was 20m/min. The changes of muscle fiber microstructures in group E were larger than those in group E. The expression of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle of group E and group L increased after exercise of different muscle contraction modes (concentric and centrifugal) at the same oxygen uptake intensity, compared with group C, and the expression of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle of group E was significantly higher than that in group C, and the expression of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle was increased compared with group C. However, there was no significant difference in the expression of PGC-1 偽 protein between the two groups, and the expression of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle was significantly higher than that in group L at 0 h or 48 h, but there was no significant difference at 6 h or 24 h. At 12 h, the expression of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle in group E was significantly higher than that in group L, and the expression of PGC-1 偽 protein in gastrocnemius muscle and soleus muscle in group E was significantly higher than that in group L at 12 h. There was significant difference at 12h ~ 48h (P 0.05A), but there was no significant difference (P 0.05) at 6h ~ 24h. Conclusion: centrifugal contraction can significantly promote the expression of PGC-1 偽 protein in skeletal muscle of rats after a single treadmill exercise with the same oxygen intake, and it lasts for a long time, showing a double peak phenomenon immediately and 48 hours after exercise. There was an increasing trend of concentric contraction, but no statistical significance.
【學(xué)位授予單位】：河北師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：G804.2

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