本文選題：力竭運(yùn)動(dòng) + 運(yùn)動(dòng)性中樞疲勞��； 參考：《南京體育學(xué)院》2015年碩士論文

【摘要】：目的:本實(shí)驗(yàn)以大鼠一次性力竭運(yùn)動(dòng)為動(dòng)物模型。了解力竭運(yùn)動(dòng)前后及恢復(fù)期不同時(shí)段大鼠紋狀體中DA含量及D1DR和D2DR相對(duì)表達(dá)量的變化情況,觀察力竭運(yùn)動(dòng)前后及恢復(fù)期紋狀體中DA含量及其受體相對(duì)表達(dá)量的變化規(guī)律,分析DA與D1DR、DA和D2DR以及D1DR和D2DR之間可能存在的相互關(guān)系,探討運(yùn)動(dòng)性疲勞發(fā)生及其24小時(shí)恢復(fù)期內(nèi)紋狀體DA及D1DR和D2DR的調(diào)控作用,為更深入研究運(yùn)動(dòng)性中樞疲勞產(chǎn)生及其恢復(fù)機(jī)制提供實(shí)驗(yàn)依據(jù)。方法:將36只SD大鼠適應(yīng)性喂養(yǎng)一周后隨機(jī)分成安靜組、力竭運(yùn)動(dòng)即刻組、力竭運(yùn)動(dòng)后3小時(shí)組、力竭運(yùn)動(dòng)后8小時(shí)組、力竭運(yùn)動(dòng)后15小時(shí)組和力竭運(yùn)動(dòng)后24小時(shí)組,每組6只,共6組。為了消除生物節(jié)律的影響,所有力竭運(yùn)動(dòng)組大鼠均在上午進(jìn)行一次性力竭游泳運(yùn)動(dòng),運(yùn)動(dòng)結(jié)束后于相應(yīng)時(shí)間點(diǎn)斷頭取腦,冰面上剝離紋狀體。安靜組與力竭運(yùn)動(dòng)組大鼠同一時(shí)間采樣。樣品采集完成后用ELISA測(cè)試盒測(cè)試大鼠紋狀體DA含量,用Western Blot檢測(cè)大鼠紋狀體D1DR和D2DR的相對(duì)表達(dá)量。結(jié)果:1、力竭運(yùn)動(dòng)恢復(fù)期大鼠紋狀體DA含量的變化:與安靜組相比,力竭運(yùn)動(dòng)即刻組大鼠紋狀體DA含量水平顯著性下降(p0.001),力竭運(yùn)動(dòng)后恢復(fù)8小時(shí)、15小時(shí)、24小時(shí)大鼠紋狀體DA含量顯著增加(p0.001);且力竭運(yùn)動(dòng)恢復(fù)15小時(shí)達(dá)到峰值;與力竭運(yùn)動(dòng)即刻組相比,恢復(fù)3小時(shí)、8小時(shí)、15小時(shí)、24小時(shí)大鼠紋狀體DA含量水平均顯著性升高且p0.0001。2、力竭運(yùn)動(dòng)恢復(fù)期大鼠紋狀體D1DR相對(duì)表達(dá)量變化:與安靜組相比,力竭運(yùn)動(dòng)即刻組大鼠紋狀體D1DR相對(duì)含量顯著性降低(p0.001),力竭運(yùn)動(dòng)3小時(shí)組、8小時(shí)組、15小時(shí)組、24小時(shí)組均高于與安靜組水平,且差異具有顯著性(P0.001);與力竭運(yùn)動(dòng)即刻組相比,力竭運(yùn)動(dòng)后恢復(fù)3小時(shí)組、8小時(shí)組、15小時(shí)組和24小時(shí)組大鼠紋狀體D1DR相對(duì)表達(dá)量均有所增加且P0.001。3、力竭運(yùn)動(dòng)恢復(fù)期大鼠紋狀體D2DR相對(duì)表達(dá)量的變化:與安靜組相比,力竭運(yùn)動(dòng)即刻組大鼠紋狀體D2DR相對(duì)表達(dá)量明顯下降(P0.001),恢復(fù)8小時(shí)達(dá)到24小時(shí)恢復(fù)期的最高值(p0.05),隨后15小時(shí)開(kāi)始下降接近于安靜組水平;與力竭運(yùn)動(dòng)后即刻組相比,力竭運(yùn)動(dòng)恢復(fù)3小時(shí)組、8小時(shí)組、15小時(shí)組和24小時(shí)組大鼠紋狀體D2DR的相對(duì)表達(dá)量均出現(xiàn)增加且p0.001。4、力竭運(yùn)動(dòng)恢復(fù)期大鼠紋狀體DA含量與D1DR相對(duì)表達(dá)量存在高度相關(guān)關(guān)系(r=0.71)且具有統(tǒng)計(jì)學(xué)意義(p0.001);DA含量與D2DR表達(dá)量之間的相關(guān)性較低弱(r=0.32),無(wú)統(tǒng)計(jì)學(xué)意義(p0.05);D1DR和D2DR之間的存在高度正相關(guān)關(guān)系(r=0.83,p0.001)。結(jié)論:1、紋狀體中DA具有雙向調(diào)節(jié)作用。當(dāng)運(yùn)動(dòng)至力竭后紋狀體DA含量降低可通過(guò)間接通路抑制機(jī)體的運(yùn)動(dòng)能力,參與運(yùn)動(dòng)性中樞疲勞的發(fā)生;在24小時(shí)恢復(fù)期內(nèi)DA含量達(dá)到峰值后緩慢下降但仍顯著高于安靜水平,可激活直接通路活性,使其恢復(fù)到正常水平,從而使中樞興奮性逐漸加強(qiáng),機(jī)體的運(yùn)動(dòng)能力得以保持甚至提高。2、運(yùn)動(dòng)至力竭時(shí),紋狀體D1DR和D2DR相對(duì)表達(dá)量均顯著低于安靜時(shí)水平,有利于保護(hù)神經(jīng)元免受興奮毒性的損害,從而起到機(jī)體保護(hù)性抑制的作用。在恢復(fù)期紋狀體D1DR和D2DR相對(duì)表達(dá)量上調(diào),可激活間接通路影響錐體外系實(shí)現(xiàn)對(duì)機(jī)體運(yùn)動(dòng)功能的調(diào)控,使機(jī)體的疲勞程度減輕。3、在運(yùn)動(dòng)疲勞產(chǎn)生及其恢復(fù)過(guò)程中,大鼠紋狀體DA含量的增加可刺激D1DR的表達(dá),而D2DR表達(dá)量主要受D1DR表達(dá)量介導(dǎo)。
[Abstract]:Objective: To investigate the changes of the content of DA and the relative expression of D1DR and D2DR in the striatum of rats before and after the exhaustion exercise, and to observe the changes of the content of DA and the relative expression of the receptor in the striatum before and after the exhaustion exercise, and to analyze the DA and D1DR, DA, DA. The possible interaction between D2DR and D1DR and D2DR and the regulation of the striatal DA and D1DR and D2DR in the 24 hour recovery period are discussed in order to provide the experimental basis for the further study of the mechanism of sports central fatigue and its recovery mechanism. Methods: 36 SD rats were fed for one week and randomly divided into silence. Group, exhausted exercise immediate group, 3 hours after exhaustive exercise group, 8 hours after exhaustive exercise, 15 hours after exhaustion exercise and 24 hours after exhaustive exercise, 6 in each group, 6 groups. In order to eliminate the influence of biological rhythm, all the rats in the exhausted exercise group were all exhausted swimming in the morning, after the exercise ended at the corresponding time point. Take the brain and peel the striatum on the ice surface. The rats in the quiet group and the exhaustive exercise group were sampled at the same time. After the sample collection was completed, the DA content of the striatum was measured by ELISA test box, and the relative expression of D1DR and D2DR in the striatum of the rats was detected by Western Blot. Results: 1, the changes in the DA content in the striatum of the exhaustive exercise rats were compared with those in the quiet group. The level of DA in the striatum of rats was significantly decreased (p0.001), after exhaustive exercise, the content of DA in the striatum was significantly increased (p0.001) in 24 hours after exhaustive exercise (p0.001), and the exhaustion movement recovered for 15 hours and reached the peak value, compared with the immediate group of exhaustive exercise, 3 hours, 8 hours, 15 hours, and 24 hours of DA in the striatum of rats. The mean significant increase of water volume and p0.0001.2, the relative expression of D1DR in the striatum of the rats in the recovery period of exhaustion: compared with the quiet group, the relative content of D1DR in the striatum was significantly lower (p0.001), the 3 hour group of exhaustive exercise, the 8 hour group, the 15 hour group, and the 24 hour group were all higher than those in the quiet group, and the difference had the difference. Significance (P0.001); compared with the immediate group of exhaustive exercise, 3 hours after exhaustive exercise, 8 hour group, 15 hour group and 24 hour group, the relative expression of D1DR in the striatum of rats increased and P0.001.3, the relative expression of D2DR in the striatum of the exhausted exercise rats was changed: compared with the quiet group, the striatum was D in the immediate group of exhaustive exercise group. The relative expression of 2DR decreased significantly (P0.001) and reached the highest value of the 24 hour recovery period of 8 hours (P0.05), and then decreased close to the level of the quiet group after 15 hours. The relative expression of D2DR in the striatum of the 15 hour group and the 24 hour group increased in the 8 hour group compared with the immediate group after the exhaustion movement. And p0.001.4, there was a high correlation between the DA content in the striatum and the relative expression of D1DR (r=0.71) in the recovery period of exhaustive exercise (r=0.71) and had statistical significance (p0.001), and the correlation between the content of DA and the expression of D2DR was low (r=0.32), and there was no statistical significance (P0.05), and there was a highly positive correlation between D1DR and D2DR (r=0.83,). 1, DA in striatum has a bidirectional regulation effect. The decrease of DA content in striatum after exercise to exhaustion can inhibit the exercise ability of the body through indirect pathway and participate in the occurrence of motor central fatigue. In the 24 hour recovery period, the content of DA decreases slowly but is still significantly higher than the quiet level, which can activate the direct pathway activity and make it restorable. At the normal level, the central excitability is gradually strengthened and the motor ability of the body can be maintained or even increased by.2. When the movement to exhaustion, the relative expression of D1DR and D2DR in the striatum is significantly lower than that in the quiet time. It is beneficial to protect the neurons from the damage of the excitotoxicity and thus play the role of protective inhibition in the body. The relative expression of D1DR and D2DR is up-regulated, which can activate the indirect pathway to regulate the exercise function of the body by the extrapyramidal system and reduce the fatigue degree of the body by.3. The increase of the DA content in the striatum of the rat can stimulate the expression of D1DR, while the expression of D2DR is mainly mediated by the expression of D1DR in the process of movement fatigue and recovery.

【學(xué)位授予單位】：南京體育學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：G804.7

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