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解脲支原體金標(biāo)免疫層析檢測(cè)試劑盒的研制

發(fā)布時(shí)間:2019-02-28 12:12
【摘要】:目的研制一種解脲支原體(Uu)膠體金免疫層析試劑盒,用于臨床快速檢測(cè)、流行病學(xué)調(diào)查。方法原核表達(dá)解脲支原體MB(Uu-MB)蛋白,制備Uu-MB單抗后膠體金標(biāo)記,采用雙抗體夾心法制備試劑盒,評(píng)價(jià)試劑盒的特異性、敏感性、穩(wěn)定性及符合率。結(jié)果對(duì)Uu陽(yáng)性培養(yǎng)物和Uu-MB具有良好特異性;37℃放置12d質(zhì)量穩(wěn)定;試劑盒與(Uu)支原體分離鑒別管的檢測(cè)陽(yáng)性率分別為29.59%和71.42%;Uu金標(biāo)檢測(cè)試劑盒與Uu分離鑒別管的陽(yáng)性、陰性符合率為41.28%、100.0%;Uu分離鑒別管培養(yǎng)物經(jīng)PCR鑒定,兩者的陽(yáng)性、陰性符合率為100%、75.65%。結(jié)論研制的試劑盒操作簡(jiǎn)便,具有特異性強(qiáng)、敏感性高等特點(diǎn),是一種適宜普及推廣的Uu感染檢測(cè)產(chǎn)品。
[Abstract]:Objective to develop a gold immunochromatographic kit for rapid detection and epidemiological investigation of Ureaplasma Urealyticum (Uu) colloid. Methods Ureaplasma Urealyticum MB (Uu-MB) protein was expressed in E. coli and labeled with colloidal gold after preparation of Uu-MB monoclonal antibody. The kit was prepared by double antibody sandwich method. The specificity, sensitivity, stability and coincidence rate of the kit were evaluated. Results there was a good specificity for Uu positive culture and Uu-MB, the quality was stable at 37 鈩,

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