【摘要】：目的研究褪色沙雷菌的耐藥率與分子流行病學規(guī)律,建立多位點序列分析方法,明確其進化特征,為臨床針對性使用抗菌藥物和防控此致病菌感染提供依據(jù)。方法對2013年9月-2014年12月分離的40株褪色沙雷菌進行體外藥物敏感性試驗與多位點序列分析(MLSA),并用Splits tree與CLUSTALW軟件進行生物信息學分析,揭示其流行趨勢。結果體外藥敏試驗結果表明,40株褪色沙雷菌對13種抗菌藥物的耐藥率在10.0%~52.5%,對亞胺培南耐藥率為32.5%;MLSA設計4個管家基因,GC含量約為60.0%;各等位基因數(shù)分布于14~16,各多態(tài)性位點數(shù)分布于33~74,gyrB的多態(tài)性位點數(shù)最多(74個);Splits tree軟件分裂分解分析結果顯示,大部分褪色沙雷菌聚在一起,形成一個克隆復合體;CLUSTALW軟件聚類分析結果顯示,40株褪色沙雷菌形成19個ST型,其中ST8型12株,且ST8型是耐碳青霉烯類抗菌藥物褪色沙雷菌的流行與暴發(fā)型,ST8、ST9、ST10與ST11形成一個克隆復合體CC8。結論褪色沙雷菌對碳青霉烯類抗菌藥物耐藥率較高,且多為泛耐藥菌株;褪色沙雷菌的分子流行病學趨勢相對較慢,在基因組上高度保守,目前需要重點監(jiān)測以ST8型為代表褪色沙雷菌CC8克隆復合體的流行,以防其在醫(yī)院的大規(guī)模流行與暴發(fā)。
[Abstract]:Objective to study the drug resistance rate and molecular epidemiology of Shareh discoloration, to establish a multilocus sequence analysis method, to identify its evolutionary characteristics, and to provide evidence for clinical use of antimicrobial agents and prevention and control of this pathogen infection. Methods 40 strains of Shareh discoloration isolated from September 2013 to December 2014 were tested for in vitro drug sensitivity and multisite sequence analysis (MLSA),) and bioinformatics analysis with Splits tree and CLUSTALW software were carried out to reveal the epidemic trend. Results the results of in vitro drug sensitivity test showed that the resistance rate of 40 strains of Shareh discoloration to 13 antimicrobial agents was 10.0% and 32.5%, respectively. The resistance rate to imipenem was 32.5% and 4 housekeeping genes were designed, and the GC content was about 60.0%. The number of alleles was 14 / 16, and the number of polymorphic loci was the highest among the polymorphic loci of 3374gyrB (74); Splits tree software cleavage analysis showed that the majority of Shareh discoloring bacteria gathered together to form a clone complex; The results of cluster analysis by CLUSTALW software showed that 40 strains of Shareh discoloration bacteria formed 19 ST type, of which 12 were ST8 type, and ST8 type was the epidemic and violent hairstyle of Shareh bacillus resistant to carbapenem antibiotics. Formation of a clone complex CC8. by ST10 and ST11 Conclusion the resistance rate of Shareh discoloration to carbapenem antibiotics is high, and most of them are pan-resistant strains. The molecular epidemiological trend of Shareh fading bacteria is relatively slow and highly conserved in the genome. It is necessary to monitor the prevalence of the CC8 clone complex of Shareh discoloring bacteria represented by ST8 type in order to prevent its large-scale epidemic and outbreak in hospitals.
【作者單位】： 蘇州大學附屬第一醫(yī)院檢驗科;
【基金】：國家自然科學青年基金資助項目(81501425) 江蘇省衛(wèi)生廳醫(yī)學科研基金資助項目(Q201401) 江蘇省研究生培養(yǎng)創(chuàng)新工程基金資助項目(KYLX-1261) 蘇州市“科教興衛(wèi)”青年基金資助項目(kjxw2014008)
【分類號】：R446.5;R440

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