【摘要】：隨著高通量測序技術和蛋白組學技術的發(fā)展,越來越多的病原菌新基因被發(fā)現(xiàn),研究病原菌新基因有助于更好的防控由該病原菌引起的疾病的傳播。本文從生物信息學角度、新基因功能獲得與缺失、與新基因編碼產物互作蛋白的分析等方面綜述了適用于病原菌新基因功能研究的策略,為基因分子生物學等相關領域提供參考。此外,本文還結合常規(guī)的新基因研究方法,將芯片技術,2D-DIGE技術滲透入病原菌新基因研究策略中,使研究者能更快捷準確的找出新基因功能研究的切入點,進而制定切實可行的新基因功能的研究方案。
[Abstract]:With the development of high-throughput sequencing and proteomics, more and more new genes of pathogenic bacteria have been found. In this paper, the strategies suitable for the study of new gene function of pathogenic bacteria were reviewed from the aspects of bioinformatics, function acquisition and deletion of new genes, and analysis of interaction proteins with new gene encoding products. To provide reference for gene molecular biology and other related fields. In addition, combined with the conventional new gene research methods, the microarray technology and 2D-DIGE technology are integrated into the new gene research strategy of pathogenic bacteria, so that the researchers can find out the breakthrough point of the new gene function research more quickly and accurately. Furthermore, a feasible research program of new gene function is established.
【作者單位】： 揚州大學江蘇省人獸共患病學重點實驗室 江蘇省動物重要疫病與人獸共患病防控協(xié)同創(chuàng)新中心;
【基金】：國家自然科學基金(No.31372449) 國家支撐計劃(2014BAD13B02)~~
【分類號】：R440

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本文編號：2320201