我國部分地區(qū)獻血人群中單純皰疹病毒感染流行情況
發(fā)布時間：2018-08-20 13:14
【摘要】：目的了解中國獻血人群中單純皰疹病毒(HSV)的流行率、病毒載量及抗體檢出情況。方法構(gòu)建的實時熒光定量PCR體系,檢測來自重慶、洛陽、烏魯木齊、綿陽和柳州等5個采供血機構(gòu)3 794(人)份獻血者血漿標本的HSV DNA,并測定DNA陽性標本的病毒載量;同時隨機抽取其中292(人)份標本用ELISA法檢測Ig G和Ig M抗體的感染情況。結(jié)果在本組獻血者人群中,發(fā)現(xiàn)43份HSV DNA反應(yīng)性標本,占1.13%(43/3 794),5個采供血機構(gòu)的檢出率分別為重慶1.24%(20/1611)、洛陽0(0/624)、烏魯木齊3.63%(14/413)、綿陽1.88%(9/479)和柳州0(0/667);病毒載量(9.15×102-9.65×104)(中位數(shù)3.53×103)cp/m L。292份經(jīng)ELISA檢測的標本中,Ig G抗體陽性率16.44%(48/292),Ig M抗體陽性率4.45%(13/292),Ig G、Ig M抗體雙陽性率2.40%(7/292)。結(jié)論目前HSV在我國獻血人群中存在一定的流行率,但是檢測出的HSV DNA陽性標本的病毒載量較低;是否需要對特殊受血者做血液HSV檢測值得進一步探討。
[Abstract]:Objective To investigate the prevalence, viral load and antibody detection of herpes simplex virus (HSV) in Chinese blood donors. Methods A real-time fluorescence quantitative PCR system was established to detect the HSV DNA in 3 794 blood donors from 5 blood donation institutions including Chongqing, Luoyang, Urumqi, Mianyang and Liuzhou, and to detect the disease of DNA positive samples. Results 43 HSV DNA reactive specimens (43/3 794) were found in this group of blood donors. The detection rates of 5 blood donors were 1.24% (20/1611) in Chongqing, 0 (0/624) in Luoyang, 3.63% (14/413) in Urumqi, 1.8% in Mianyang and 3.13% (14/413) in Urumqi, respectively. 8% (9/479) and Liuzhou 0 (0/667); viral load (9.15 *102-9.65 *104) (median 3.53 *103) cp/m L.292 samples tested by ELISA, the positive rate of Ig G antibody was 16.44% (48/292), the positive rate of Ig M antibody was 4.45% (13/292), the double positive rate of Ig G and Ig M antibody was 2.40% (7/292). Conclusion There was a certain prevalence of HSV in Chinese blood donors at present. The viral load of HSV DNA positive specimens was low, and whether it was necessary to detect HSV in blood of special recipients should be further explored.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院輸血研究所;
【分類號】：R440
[Abstract]:Objective To investigate the prevalence, viral load and antibody detection of herpes simplex virus (HSV) in Chinese blood donors. Methods A real-time fluorescence quantitative PCR system was established to detect the HSV DNA in 3 794 blood donors from 5 blood donation institutions including Chongqing, Luoyang, Urumqi, Mianyang and Liuzhou, and to detect the disease of DNA positive samples. Results 43 HSV DNA reactive specimens (43/3 794) were found in this group of blood donors. The detection rates of 5 blood donors were 1.24% (20/1611) in Chongqing, 0 (0/624) in Luoyang, 3.63% (14/413) in Urumqi, 1.8% in Mianyang and 3.13% (14/413) in Urumqi, respectively. 8% (9/479) and Liuzhou 0 (0/667); viral load (9.15 *102-9.65 *104) (median 3.53 *103) cp/m L.292 samples tested by ELISA, the positive rate of Ig G antibody was 16.44% (48/292), the positive rate of Ig M antibody was 4.45% (13/292), the double positive rate of Ig G and Ig M antibody was 2.40% (7/292). Conclusion There was a certain prevalence of HSV in Chinese blood donors at present. The viral load of HSV DNA positive specimens was low, and whether it was necessary to detect HSV in blood of special recipients should be further explored.
【作者單位】： 中國醫(yī)學(xué)科學(xué)院北京協(xié)和醫(yī)學(xué)院輸血研究所;
【分類號】：R440
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