本文選題：快速動(dòng)眼睡眠剝奪 + 抑郁樣行為��； 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2016年05期

【摘要】：目的探討連續(xù)長(zhǎng)時(shí)間快速動(dòng)眼睡眠剝奪(5 d)對(duì)C57BL/6J小鼠抑郁樣行為和杏仁核單胺氧化酶A水平的影響。方法成年雄性C57BL/6J小鼠隨機(jī)分成空白組、對(duì)照組和快速動(dòng)眼睡眠剝奪組,通過多小平臺(tái)水環(huán)境法建立快速動(dòng)眼睡眠剝奪模型。睡眠剝奪結(jié)束后,于不同時(shí)間點(diǎn)采用曠場(chǎng)實(shí)驗(yàn)檢測(cè)各組運(yùn)動(dòng)能力,強(qiáng)迫游泳實(shí)驗(yàn)和糖水偏好實(shí)驗(yàn)檢測(cè)各組抑郁樣行為。之后,取腦分離杏仁核,利用Western Blot和Real-time PCR檢測(cè)各組單胺氧化酶A的表達(dá)水平。結(jié)果快速動(dòng)眼睡眠剝奪后的1~3 d內(nèi),睡眠剝奪小鼠運(yùn)動(dòng)能力明顯下降,第4天可恢復(fù)到正常水平;第7~14天強(qiáng)迫游泳實(shí)驗(yàn)顯示小鼠不動(dòng)時(shí)間延長(zhǎng);糖水偏好率也明顯降低。Western blot和Real-time PCR檢測(cè)發(fā)現(xiàn)杏仁核中單胺氧化酶A表達(dá)水平明顯升高。結(jié)論連續(xù)長(zhǎng)時(shí)間快速動(dòng)眼睡眠剝奪導(dǎo)致小鼠遲發(fā)性抑郁樣行為,該現(xiàn)象很可能與杏仁核單胺氧化酶水平變化有關(guān)。
[Abstract]:Objective to investigate the effects of continuous rapid eye movement sleep deprivation (5 d) on depressive behavior and amygdala monoamine oxidase A level in C57BL / 6J mice. Methods Adult male C57BL / 6J mice were randomly divided into three groups: blank group, control group and REM sleep deprivation group. At different time points after sleep deprivation, open field test was used to test the exercise ability of each group, forced swimming test and sugar water preference test were used to detect depressive behavior in each group. After that, the amygdala was isolated and the expression of monoamine oxidase A was detected by Western Blot and Real-time PCR. Results within 1 to 3 days after rapid eye movement sleep deprivation, the motor ability of sleep deprived mice decreased significantly and returned to normal level on the 4th day, and the forced swimming test on day 714 showed that the immobility time of mice was prolonged. The expression of monoamine oxidase A in amygdala was significantly increased by Western blot and Real-time PCR. Conclusion prolonged rapid eye movement sleep deprivation may lead to delayed depressive behavior in mice, which may be related to the changes of monoamine oxidase level in amygdala.
【作者單位】： 南方醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院神經(jīng)生物學(xué)教研室;
【基金】：國(guó)家自然科學(xué)基金(313711484)~~
【分類號(hào)】：R740

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