本文選題：基質(zhì)輔助激光解吸電離飛行時(shí)間質(zhì)譜 + 微生物　； 參考：《中國(guó)老年學(xué)雜志》2016年13期

【摘要】：正基質(zhì)輔助激光解吸電離飛行時(shí)間質(zhì)譜(MALDI-TOF)是一種新型的質(zhì)譜技術(shù),具有靈敏度高,樣品用量少,分析速度快的特點(diǎn)。目前,MALDI-TOF已開始應(yīng)用于多項(xiàng)微生物的檢測(cè),為臨床疾病的診療提供有效的指導(dǎo)。1 MALDI-TOF鑒定微生物的特點(diǎn)及優(yōu)勢(shì)MALDI-TOF系統(tǒng)由基質(zhì)輔助激光解吸電離離子源(MALDI)、飛行時(shí)間質(zhì)量分析器(TOF)和檢測(cè)器組成,屬于一種軟電離技術(shù),適用于混合物及生物大分子的測(cè)定〔1〕。其原理是利用激光照射待測(cè)物與基質(zhì)形成的共結(jié)晶薄膜,通過基質(zhì)吸收能量并將能量轉(zhuǎn)移至待測(cè)物分子,使分子解離成離子,利用離子在
[Abstract]:Positive matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF) is a new type of mass spectrometry technology with high sensitivity, small sample consumption and fast analysis speed. At present, MALDI-TOF has been applied to the detection of many microbes. To provide effective guidance for the diagnosis and treatment of clinical diseases. 1. The characteristics and advantages of MALDI-TOF in the identification of microorganisms. The MALDI-TOF system is composed of matrix assisted laser desorption ionization ion source (MALDI), time of flight quality analyzer (TOF) and detector. It belongs to a soft ionization technology. Suitable for the determination of mixtures and biomolecules (1). The principle is to use laser to irradiate the eutectic film formed between the tested object and the matrix, absorb the energy through the matrix and transfer the energy to the molecule to be tested, so that the molecule can be dissociated into ions.
【作者單位】： 吉林大學(xué)第一醫(yī)院檢驗(yàn)科;吉林大學(xué)第一醫(yī)院神經(jīng)腫瘤外科;
【分類號(hào)】：R446.5
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本文編號(hào)：2052250