發(fā)布時間：2018-06-01 07:27

  本文選題：肺炎克雷伯菌 + 噬菌體�。� 參考：《中國病原生物學(xué)雜志》2016年01期

【摘要】：目的研究肺炎克雷伯菌噬菌體LH-02的生物學(xué)特性及基因組性質(zhì)。方法觀察LH-02的噬菌斑形態(tài)和電鏡下形態(tài);調(diào)查LH-02的噬菌譜,并觀察其一步生長曲線以及理化穩(wěn)定性;提取噬菌體基因組進行酶切鑒定。結(jié)果LH-02在宿主菌KF29菌苔上形成直徑2~3mm的透明噬菌斑,外周有寬2~3mm的半透明暈環(huán);電鏡觀察確定其為有尾噬菌體目,短尾噬菌體科;一步生長曲線顯示LH-02感染KF29的潛伏期短(約15min),裂解量大(約217PFU/細胞);LH-02在一個較寬的pH范圍內(nèi)(5~10)和較高的溫度(50℃)范圍內(nèi)活性穩(wěn)定;LH-02的核酸可被KpnⅠ,EcoRⅠ,HindⅢ降解,但不能被BamHⅠ降解。結(jié)論 LH-02為裂解性噬菌體,基因組為雙鏈DNA,其潛伏期短,裂解量大,穩(wěn)定性好,有望成為生物抗菌劑。
[Abstract]:Objective to study the biological and genomic properties of Klebsiella pneumoniae phage LH-02. Methods the plaque morphology and electron microscope morphology of LH-02 were observed, the phage spectrum of LH-02 was investigated, the one-step growth curve and physicochemical stability were observed, the phage genome was extracted and identified by enzyme digestion. Results LH-02 formed transparent plaque with diameter 2~3mm on the coating of host strain KF29, and had a semitransparent halo ring with wide 2~3mm around it. The one-step growth curve showed that the incubation period of LH-02 infection with KF29 was short (about 15 mins), and the nucleic acid with stable activity of LH-02 could be degraded by Kpn 鈪，

本文編號：1963366