本文選題：喹諾酮 + gyrA基因��； 參考：《中國(guó)人獸共患病學(xué)報(bào)》2016年10期

【摘要】：目的為闡明寧波地區(qū)耐多藥結(jié)核分枝桿菌(Multiple drug-resistant tuberculosis,MDR-TB)的gyr基因突變特征,深入研究MDR-TB對(duì)喹諾酮類藥物耐藥與gyr基因突變特征的關(guān)系。方法采用1%比例法對(duì)MDR-TB進(jìn)行氧氟沙星藥敏檢測(cè)實(shí)驗(yàn),通過(guò)DNA直接測(cè)序法分析MDR-TB的gyr基因突變情況。結(jié)果 120株MDR-TB臨床分離株中有34株對(duì)喹諾酮耐藥,總耐藥率為28.33%(34/120)。34株耐喹諾酮菌株中,30株gyr基因發(fā)生突變,突變率為88.24%(30/34)。30株gyr基因發(fā)生突變的菌株中g(shù)yrA基因突變有29株,占96.67%(29/30),突變位點(diǎn)包括90、91和94位氨基酸;gyrB基因突變有2株,其中1株均合并gyrA基因突變,占6.67%(2/30),突變位點(diǎn)包括499和502位氨基酸。結(jié)論寧波地區(qū)MDRTB對(duì)喹諾酮類藥物耐藥形勢(shì)較為嚴(yán)峻,gyrA基因突變與MDR-TB對(duì)喹諾酮類藥物耐藥相關(guān)。
[Abstract]:Objective to study the relationship between MDR-TB resistance to quinolones and gyr gene mutation in Ningbo area in order to elucidate the characteristics of gyr gene mutation in multidrug resistant Mycobacterium tuberculosis (MDR-TB). Methods 1% ratio method was used to detect the susceptibility of MDR-TB to ofloxacin and the mutation of gyr gene in MDR-TB was analyzed by DNA direct sequencing. Results among the 120 clinical isolates of MDR-TB, 34 were resistant to quinolones, and the total resistance rate was 28.33%. Among the 34 / 120 / 34 quinolone-resistant strains, 30 gyr genes were mutated, and the mutation rate was 88.24% 30% 34 / 30 gyr gene mutation in 29 strains of gyrA gene. There were two mutations in 90.91 and 94 amino acid gyrB genes, one of which was combined with gyrA gene mutation, which accounted for 6.6770 / 30, and the mutation sites included 499 and 502 amino acids. Conclusion MDRTB resistance to quinolones is more severe in Ningbo area. The mutation of gyra gene is associated with MDR-TB resistance to quinolones.
【作者單位】： 浙江省寧波市疾病預(yù)防控制中心結(jié)核病防制所;
【基金】：寧波市自然科學(xué)基金(No.2015A610195) 浙江省公共衛(wèi)生應(yīng)急檢測(cè)關(guān)鍵技術(shù)重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金資助~~
【分類號(hào)】：R440
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本文編號(hào)：1881024