本文選題：量子點　切入點：免疫熒光分析　出處：《華東師范大學學報(自然科學版)》2016年02期 　論文類型：期刊論文

【摘要】：通過制備量子點熒光檢測探針,構建了一種基于量子點探針和免疫磁珠的蛋白質檢測方法,實現了對蛋白腫瘤標志物癌胚抗原(carcinoembryonic antigen,CEA)的高靈敏定量檢測.在該檢測體系中,若存在有靶標蛋白,其與量子點檢測探針以及捕獲探針之間會發(fā)生免疫反應形成三明治結構,利用磁力分離器對免疫復合物進行富集后,通過檢測富集在磁珠表面的量子點熒光信號,可實現對靶標蛋白的定量.該方法的檢測靈敏度為38 pg/mL,線性范圍為0.39~50ng/mL,臨床質控樣本檢驗結果表明,該方法準確度高,可重復性好,可應用于臨床樣本檢測.該量子點探針檢測體系具有靈敏度高、特異性好、樣品消耗量低等優(yōu)點,在疾病早期診斷方面具有廣闊的應用前景.
[Abstract]:A method of protein detection based on quantum dot probe and immunomagnetic beads was constructed by preparing quantum dot fluorescence detection probe. A highly sensitive and quantitative detection method for carcinoembryonic antigen (CEA), a protein tumor marker, was developed. If there is a target protein, the immune reaction between the target protein and the quantum dot detection probe and the capture probe will form a sandwich structure, and the immune complex can be enriched by magnetic separator. The quantification of target protein can be achieved by detecting the fluorescence signals of quantum dots enriched on the magnetic bead surface. The sensitivity of the method is 38 PG / mL and the linear range is 0.39 ~ 50 ng / mL. The results of clinical quality control samples show that the method is accurate and reproducible. The quantum dot probe detection system has the advantages of high sensitivity, good specificity and low sample consumption, so it has a broad application prospect in the early diagnosis of disease.
【作者單位】： 華東師范大學生命科學學院;中國科學院上海微系統(tǒng)與信息技術研究所傳感技術聯(lián)合國家重點實驗室;
【基金】：國家重點基礎發(fā)展計劃(2012CB933303) 國家自然科學基金(81101645;61271162;81472751;21405167) 上海市科委項目(12nm0503702;11JC1414400;BRC2012002) 東南大學生物電子學國家重點實驗室開放研究項目
【分類號】：R446.1;O657.3

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