本文關(guān)鍵詞： 膿毒癥 n-多不飽和脂肪酸 鞘氨醇激酶 磷酸鞘氨醇受體　出處：《腸外與腸內(nèi)營(yíng)養(yǎng)》2016年05期 　論文類型：期刊論文

【摘要】：目的:觀察富含n-3多不飽和脂肪酸(n-3 PUFA)的腸外營(yíng)養(yǎng)(PN)能否通過(guò)鞘氨醇激酶1(Sph K1)和磷酸鞘氨醇受體(S1P)途徑影響機(jī)體的炎性反應(yīng)。方法:對(duì)大鼠實(shí)施頸外靜脈置管,2 d后行盲腸結(jié)扎穿孔,然后隨機(jī)分為等滲鹽水組(靜脈輸注等滲鹽水,n=20)、魚(yú)油組(魚(yú)油+PN,n=20)和大豆油組(PN,n=20),隨后行PN支持5 d。另設(shè)正常對(duì)照組(n=10)實(shí)施假手術(shù)。檢測(cè)血清IL-10和高遷移率族蛋白(HMGB-1)濃度、脾組織中Sph K1、S1PR1、S1PR3表達(dá)情況以及Sph K1酶活性。結(jié)果:與對(duì)照組比,等滲鹽水組大鼠Sph K1 mRNA、蛋白表達(dá)和酶活性明顯升高,S1PR3 mRNA和蛋白表達(dá)也上調(diào);魚(yú)油組血清IL-10和HMGB-1濃度顯著降低;Sph K1 mRNA、蛋白表達(dá)和酶活性顯著受抑制;S1PR3 mRNA和蛋白表達(dá)也受抑。結(jié)論:n-3 PUFA的PN可通過(guò)抑制Sph K1和S1PR3通路改善膿毒癥大鼠的炎性反應(yīng)。
[Abstract]:Objective: to observe whether PNs rich in n-3 polyunsaturated fatty acids (n-3 PUFAs) can affect the inflammatory response through sphingosine kinase 1 (SPH K1) and sphingosine phosphate receptor (S1P). Methods: the external jugular vein cannulation of rats was used to observe whether PNs could affect the inflammatory response of the body through sphingosine kinase 1 (SPH K1) and sphingosine phosphate receptor (S1P). D followed by cecal ligation and perforation, Then they were randomly divided into two groups: isosmotic saline group (intravenous infusion of isosmotic saline), fish oil group (fish oil group) and soybean oil group, then PN support group for 5 days, and normal control group for 5 days. Serum IL-10 and high mobility group protein (HMGB-1) were detected. Results: compared with the control group, the expression of Sph K1 mRNAs, protein expression and enzyme activity of S1PR3 mRNA and protein were significantly increased in Isotonic saline group. The concentration of IL-10 and HMGB-1 in serum of fish oil group significantly decreased SPH K1 mRNAs, and the protein expression and enzyme activity were significantly inhibited. Conclusion the PN of 1: n-3 PUFA can improve the inflammatory response of sepsis rats by inhibiting Sph K1 and S1PR3 pathway.
【作者單位】： 南京大學(xué)醫(yī)學(xué)院附屬金陵醫(yī)院(南京軍區(qū)南京總醫(yī)院)解放軍普通外科研究所;
【基金】：南京軍區(qū)醫(yī)學(xué)科技創(chuàng)新課題(12Z27)
【分類號(hào)】：R459.7
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本文編號(hào)：1522878