發(fā)布時間：2018-01-08 06:05

  本文關(guān)鍵詞：液相色譜-動態(tài)串聯(lián)質(zhì)譜技術(shù)同時檢測人尿中120種刺激劑和利尿劑等禁用物質(zhì)　出處：《中國運動醫(yī)學(xué)雜志》2016年05期 　論文類型：期刊論文

  更多相關(guān)文章： 液相色譜-動態(tài)串聯(lián)質(zhì)譜 直接進(jìn)樣 刺激劑 利尿劑 興奮劑管制

【摘要】：目的:采用液相色譜-動態(tài)串聯(lián)質(zhì)譜技術(shù),建立和驗證同時檢測人尿樣中120種刺激劑、利尿劑和其它禁用物質(zhì)的方法。方法:尿樣經(jīng)同等體積的5%醋酸鉛水溶液沉淀蛋白質(zhì)后,上清液用動態(tài)多反應(yīng)監(jiān)測模式(d MRM)進(jìn)行檢測,并比較了傳統(tǒng)MRM與動態(tài)MRM在檢測靈敏度上的差異。結(jié)果:所篩查禁用物質(zhì)的檢測限均介于0.05~20 ng/ml之間,達(dá)到世界反興奮劑機(jī)構(gòu)對禁用物質(zhì)檢測能力的要求。在低、中和高三個濃度級別的日內(nèi)和日間精密度均小于20%,準(zhǔn)確度和基質(zhì)效應(yīng)在80%~120%之間。結(jié)論:該方法具有靈敏度高、準(zhǔn)確可靠和重現(xiàn)性好等特點。動態(tài)MRM模式只掃描某個禁用物質(zhì)保留時間附近區(qū)域,駐留時間大大增加,其檢測靈敏度遠(yuǎn)高于傳統(tǒng)MRM模式,更適合于多種禁用物質(zhì)的同時檢測。
[Abstract]:Objective: to establish and verify the simultaneous determination of 120 stimulators in human urine by liquid chromatography-dynamic tandem mass spectrometry. Methods: urine samples were precipitated with the same volume of 5% lead acetate solution and the supernatant was detected by dynamic multi-reaction monitoring mode. The detection sensitivity of traditional MRM and dynamic MRM was compared. Results: the detection limit of prohibited substances for screening was between 0.05 ng/ml and 20 ng/ml. Meet the requirements of the World Anti-Doping Agency for the ability to detect banned substances. The intra-day and inter-day precision at low, medium and high levels of concentration is less than 20%. The accuracy and matrix effect were between 80% and 120%. Conclusion: this method has high sensitivity. Dynamic MRM only scans the area near the retention time of a banned substance, and the residence time increases greatly. The detection sensitivity of dynamic MRM mode is much higher than that of traditional MRM mode. It is more suitable for simultaneous detection of many banned substances.
【作者單位】： 國家體育總局反興奮劑中心;
【基金】：國家體育總局反興奮劑中心科研項目(2013ZR002)
【分類號】：R872;R446.12
【正文快照】： 刺激劑和利尿劑類藥物是競技體育中經(jīng)常被濫用的興奮劑。刺激劑通過對中樞神經(jīng)系統(tǒng)的選擇性作用,能增強機(jī)體活力和敏捷性,還可使運動員疲勞感減輕,自信心增強,注意力集中。利尿劑可促進(jìn)腎臟排尿功能,具有快速減輕體重的作用,主要用于需要控制體重的運動項目如拳擊、摔跤、舉重，

本文編號：1395903