本文關鍵詞：Bruker MS與VITEK-MS鑒定近平滑念珠菌復合體的性能對比　出處：《中華醫(yī)院感染學雜志》2016年10期 　論文類型：期刊論文

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【摘要】：目的評估中國醫(yī)院侵襲性真菌監(jiān)測網(CHIF-NET)10和11收集的近平滑念珠菌復合體原始鑒定、Bruker MS與VITEK MS鑒定結果。方法經中心實驗室分子生物學方法復核鑒定確認,CHIF-NET 10和11從全國22所醫(yī)院共收集到近平滑念珠菌復合體352株,包括近平滑念珠菌305株,似平滑念珠菌36株,擬平滑念珠菌11株;以復核鑒定結果為金標準,評價各單位原始常規(guī)方法鑒定結果,并評估Bruker MS和VITEK MS基質輔助激光解吸電離飛行時間質譜(MALDI-TOF MS)對近平滑念珠菌復合體的鑒定性能。結果與分子生物學鑒定結果相比,近平滑念珠菌的各醫(yī)院原始鑒定準確率為90.8%,9.2%的菌株被API 20C或VITEK-2錯誤鑒定為光滑念珠菌、季也蒙念珠菌等;常規(guī)方法無法鑒定似平滑念珠菌和擬平滑念珠菌;Bruker MS和VITEK MS對305株近平滑念珠菌鑒定準確率分別達到了98.4%和97.7%;Bruker Biotyper 3.0數據庫中有似平滑念珠菌和擬平滑念珠菌參考圖譜,但鑒定準確率僅分別為33.3%和27.3%;VITEK MS V2.0沒有兩種菌的參考圖譜而無法鑒定。結論該項研究率先在國內評估了Bruker MS和VITEK MS鑒定近平滑念珠菌復合體的性能,證明二者對于近平滑念珠菌的鑒定是比較可靠的。
[Abstract]:Objective to evaluate the Chinese hospital invasive fungal monitoring network (CHIF-NET) original c.parapsilosis complex identification of 10 and 11 were collected, Bruker MS and VITEK MS. The identification results confirmation method by the Central Laboratory of molecular biology methods review identification, CHIF-NET 10 and 11 from 22 hospitals across the country were collected 352 strains of Candida parapsilosis complex, including nearly 305 strains of Candida parapsilosis, like Candida paropsilosis in 36 strains, 11 strains of Candida to smooth; to review identification results as the gold standard, the evaluation results of conventional methods of each unit of original identification, and evaluation of Bruker MS and VITEK MS matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) on the performance appraisal of c.parapsilosis complex results. Compared with the results of molecular identification, the accuracy rate of each hospital c.parapsilosis original identification for 90.8%, 9.2% strains were identified as API 20C or VITEK-2 smooth read error Zhuhai bacteria, Candida guilliermondii; conventional methods can not be identified as Candida parapsilosis and Candida Bruker MS and quasi smooth; VITEK MS on the accuracy of identification of 305 strains of Candida parapsilosis respectively 98.4% and 97.7%; Bruker Biotyper 3 database like Candida parapsilosis and Candida parapsilosis Ni Ping reference map, but the identification accuracy rate respectively. 33.3% and 27.3%; VITEK MS V2.0 no reference map of two kinds of bacteria which could not be identified. Conclusion this study evaluated the performance of the first in the domestic Bruker MS and VITEK MS identification of Candida parapsilosis complex, proving the two identification for Candida parapsilosis is reliable.

【作者單位】： 中國醫(yī)學科學院北京協(xié)和醫(yī)院檢驗科;中國醫(yī)學科學院研究生院;
【基金】：國家自然科學基金資助項目(81572057)
【分類號】：R446.5
【正文快照】： 近年來隨著生命支持系統(tǒng)的廣泛應用,近平滑念珠菌復合體已成為念珠菌血癥第二常見的病原菌[1]。近平滑念珠菌復合體可引起多種臨床相關感染,顯著增加住院患者的病死率和醫(yī)療支出。2005年以前,近平滑念珠菌分為3種不同基因型(Ⅰ、Ⅱ和Ⅲ型)[2]。實驗室使用常規(guī)方法是不能夠將近

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